Metabolomics analysis of seminal plasma in patients with idiopathic Oligoasthenoteratozoospermia using high-resolution NMR spectroscopy

Basit Öğe Kaydı
dc.authoridKaraer, Abdullah/0000-0002-2010-6211
dc.authoridDogan, Berat/0000-0003-4810-1970;
dc.authorwosidKaraer, Abdullah/ABI-4667-2020
dc.authorwosidmumcu, akın/ABG-9283-2020
dc.authorwosidDogan, Berat/AAJ-7288-2020
dc.authorwosidTuncay, Görkem/ABI-6418-2020
dc.contributor.authorMumcu, A.
dc.contributor.authorKaraer, A.
dc.contributor.authorDogan, B.
dc.contributor.authorTuncay, G.
dc.date.accessioned2024-08-04T20:46:50Z
dc.date.available2024-08-04T20:46:50Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground Male infertility is a global health issue caused by a combination of different factors. Specialists generally rely on semen analysis to diagnose male infertility. However, it is known that diagnostic semen analysis fails to identify about 50% of male infertility disorders. Recently, metabolomics has been proven to be a powerful technique for the diagnosis of different diseases. Objective To determine whether metabolites could be used as potential biomarkers for the diagnosis of male factor infertility through comparing seminal plasma samples from infertile men with oligoasthenoteratozospermia (OAT) and samples from normozoospermic controls. Materials and methods This study utilized high-resolution H-1 NMR spectroscopy to reveal whether the metabolomic changes of seminal plasma obtained from 31 patients with oligoasthenoteratozospermia (OAT) are different from the ones obtained from 28 normozoospermic controls. Results Multivariate statistical analysis of NMR data concluded that the metabolomic profile of samples from patients with OAT exhibits statistically significant differences when compared to the controls. The differences were based on the metabolites lactate, citrate, lysine, arginine, valine, glutamine, creatinine, alpha-ketoglutaric acid, spermine, putrescine, and tyrosine. Except the tyrosine, levels of the above metabolites were significantly decreased in patients with OAT compared to the controls. The levels of citrate, choline, spermine, putrescine, alpha-ketoglutaric acid, valine, and tyrosine were significantly different (p < 5 x 10(-4)) between two groups. On the other hand, levels of lactate, creatinine, lysine, arginine, and glutamine were also statistically significant (0.001 p < 0.05). However, considering the p-values, the physiological relevance of these metabolites may be lower when compared to the others. A PLS-DA model built on the NMR data achieved 89.29% sensitivity and 93.55% specificity results in a leave-one-out cross-validation process. Discussion and conclusion H-1 NMR spectroscopy-based metabolomic analysis could be used as a diagnostic tool for the diagnosis of oligoasthenoteratozospermia.en_US
dc.identifier.doi10.1111/andr.12707
dc.identifier.issn2047-2919
dc.identifier.issn2047-2927
dc.identifier.issue2en_US
dc.identifier.pmid31520509en_US
dc.identifier.scopus2-s2.0-85073972634en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1111/andr.12707
dc.identifier.urihttps://hdl.handle.net/11616/98994
dc.identifier.volume8en_US
dc.identifier.wosWOS:000517623600025en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofAndrologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectoligoasthenoteratozoospermiaen_US
dc.subjectNMR spectroscopyen_US
dc.subjectmetabolomicsen_US
dc.subjectseminal plasmaen_US
dc.titleMetabolomics analysis of seminal plasma in patients with idiopathic Oligoasthenoteratozoospermia using high-resolution NMR spectroscopyen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu