Myrtenal Pretreatment Exerts a Protective Effect Against Renal Ischemia-Reperfusion Injury in Rats

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dc.contributor.authorBeytur, Leyla
dc.contributor.authorKorkmaz, Engin
dc.contributor.authorKose, Evren
dc.contributor.authorTaslidere, Asli
dc.contributor.authorTekin, Suat
dc.date.accessioned2026-04-04T13:37:38Z
dc.date.available2026-04-04T13:37:38Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractAcute kidney injury (AKI) is a serious condition with high mortality in intensive care units and during vascular surgeries. Renal ischemia-reperfusion injury (IRI) significantly contributes to AKI. Preclinical studies have shown that myrtenal (Myrt) has anti-inflammatory and antioxidant properties. We hypothesized that Myrt might alleviate renal damage caused by IRI through the modulation of oxidative stress and inflammatory processes. This study aimed to investigate the renoprotective potential of Myrt against IRI using biochemical evaluations and histological examinations. Forty male Sprague Dawley rats were assigned to four groups: sham, IRI, Myrt40+IRI, and Myrt80+IRI. Animals received daily intraperitoneal injections of Myrt (40-80 mg/kg) or solvent for 9 days before surgery. The sham group underwent laparotomy, while the other groups had AKI induced via bilateral renal pedicle clamping for 45 min, followed by 24 h of reperfusion. Renal function was assessed by blood urea nitrogen (BUN), creatinine, kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) levels. Inflammatory markers interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha) were measured, along with oxidative stress parameters malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH). Renal damage was evaluated histopathologically using hematoxylin and eosin staining and apoptosis was assessed via caspase-3 immunohistochemistry. In the IRI group, serum BUN and creatinine levels were significantly higher than the sham group but were reduced by Myrt pretreatment (p < 0.05). IRI also led to significant increases in MDA, KIM-1, NGAL, IL-1 beta, and TNF-alpha in kidney tissue (p < 0.05), which were notably decreased by Myrt pretreatment (p < 0.05). Myrt also restored SOD and CAT enzyme activities and GSH levels reduced by IRI (p < 0.05). Histological analysis showed Myrt significantly alleviated renal tissue damage and reduced caspase-3 immunoreactivity due to IRI (p < 0.05). The findings suggest that Myrt has a protective effect against renal IRI.
dc.description.sponsorshipThis study was supported by the Scientific Research Projects Unit of Inonu University (Project No: TDK-2023-3271). [TDK-2023-3271]; Scientific Research Projects Unit of Inonu University
dc.description.sponsorshipThis study was supported by the Scientific Research Projects Unit of Inonu University (Project No: TDK-2023-3271).
dc.identifier.doi10.1002/jbt.70202
dc.identifier.issn1095-6670
dc.identifier.issn1099-0461
dc.identifier.issue3
dc.identifier.orcid0000-0002-8365-2914
dc.identifier.pmid40025781
dc.identifier.scopus2-s2.0-86000123991
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/jbt.70202
dc.identifier.urihttps://hdl.handle.net/11616/109966
dc.identifier.volume39
dc.identifier.wosWOS:001435560000001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Biochemical and Molecular Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectacute kidney injury
dc.subjectapoptosis
dc.subjectinflammation
dc.subjectmyrtenal
dc.subjectoxidative stress
dc.titleMyrtenal Pretreatment Exerts a Protective Effect Against Renal Ischemia-Reperfusion Injury in Rats
dc.typeArticle

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