Biochemical and histopathological investigation of the protective effects of melatonin and vitamin E against the damage caused by acetamiprid in Balb-c mouse testicles at light and electron microscopic level

Basit Öğe Kaydı
dc.authoridGozukara Bag, Harika Gozde/0000-0003-1208-4072
dc.authoridTaslidere, Elif/0000-0003-1723-2556
dc.authoridZAYMAN, EMRAH/0000-0002-6578-3371
dc.authorwosidGozukara Bag, Harika Gozde/ABG-7588-2020
dc.authorwosidTaslidere, Elif/ABI-8046-2020
dc.contributor.authorZayman, Emrah
dc.contributor.authorGul, Mehmet
dc.contributor.authorErdemli, Mehmet Erman
dc.contributor.authorGul, Semir
dc.contributor.authorBag, Harika Gozukara
dc.contributor.authorTaslidere, Elif
dc.date.accessioned2024-08-04T20:51:41Z
dc.date.available2024-08-04T20:51:41Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe protective effects of melatonin (Mel) and vitamin E (Vit E) against the negative effects of acetamiprid (Acmp) on testicles, reproductive hormones, and oxidative stress parameters were investigated in the present study. A total of 50 Balb-c male mice were used in 7 groups; 6 mice in the control groups (distilled water, corn oil, ethanol), and 8 in other groups (Acmp, Acmp + Mel, Acmp + Vit E, Acmp + Vit E + Mel). After the experiment, which lasted 21 days, hematoxylin eosin (H&E), periodic acid Schiff (PAS), and caspase-3 immunohistochemical (IHC) staining was performed on the testicular tissues. Also, the tissues were examined ultrastructurally with the transmission electron microscopy (TEM). In the Acmp group, there were decreased seminiferous tubule diameter and epithelial thickness, epithelial degeneration, decreased spermatozoa in the lumen, decreased PAS-positive staining in the seminiferous epithelial basement membrane, edema in the interstitial area, and hydropic degeneration in Leydig cells. Caspase-3 immunoreactivity was higher than in the other groups. TEM examination showed degeneration in tubule cells, lysosomal accumulation in cells of the spermatogenic line, vacuolizations with myelin figures, and necrosis. Hydropic degeneration, electron-dense lipid vacuoles, and chromatolysis were evident in the Leydig cell cytoplasm. In Sertoli cells, electron-dense lysosomal deposits were noted. In biochemical terms, there were decreased tissue glutathione (GSH) and total antioxidant status (TAS), and increased malondialdehyde (MDA) and total oxidant status (TOS). Plasma luteinizing hormone (LH), follicular stimulating hormone (FSH), and testosterone levels were decreased. In the groups with melatonin, vitamin E, and both were applied together, tissue damage, and apoptotic cell death were reduced at both light microscopic and ultrastructural levels. In biochemical terms, there were decreased oxidative parameters and increased hormonal parameters. It was found that vitamin E was more effective in decreasing oxidative parameters and increasing antioxidative parameters when compared to melatonin, and hormonal parameters increased at a higher level in the Acmp + Vit E group than in all groups. As a result, it was found that exposure to Acmp caused damage to testicular tissue, induced oxidative stress in testicles, and decreased plasma LH, FSH, and testosterone levels, and although vitamin E is more effective than melatonin in preventing this damage, both are effective.en_US
dc.description.sponsorshipScientific Research Unit of Inonu University [TTU-2017-944]en_US
dc.description.sponsorshipThe necessary funding for this study was provided by the Scientific Research Unit of Inonu University with the project code TTU-2017-944.en_US
dc.identifier.doi10.1007/s11356-022-19143-9
dc.identifier.endpage47584en_US
dc.identifier.issn0944-1344
dc.identifier.issn1614-7499
dc.identifier.issue31en_US
dc.identifier.pmid35182334en_US
dc.identifier.scopus2-s2.0-85124814411en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage47571en_US
dc.identifier.urihttps://doi.org/10.1007/s11356-022-19143-9
dc.identifier.urihttps://hdl.handle.net/11616/100492
dc.identifier.volume29en_US
dc.identifier.wosWOS:000758313700014en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofEnvironmental Science and Pollution Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAcmpen_US
dc.subjectMelatoninen_US
dc.subjectVitamin Een_US
dc.subjectReproductive toxicityen_US
dc.subjectElectron microscopyen_US
dc.titleBiochemical and histopathological investigation of the protective effects of melatonin and vitamin E against the damage caused by acetamiprid in Balb-c mouse testicles at light and electron microscopic levelen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu