Böbrek iskemi-reperfüzyon modeli oluşturulan ratlarda astaksantin maddesinin otofaji üzerine etkisinin araştırılması
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2020
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İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Çalışmanın amacı böbrek iskemi reperfüzyon hasarı olan ratlarda astaksantinin otofaji üzerine etkilerinin araştırılmasıdır. Materyal ve Metot: Çalışmada 40 adet Spraque-Dawley cinsi erkek sıçan kullanıldı. Sıçanlar Grup 1 (Sham), Grup 2 (İ/R), Grup 3 (Atx 5 mg/kg+İ/R), Grup 4 (Atx 10 mg/kg+İ/R), Grup 5 (Atx 25 mg/kg+İ/R) olmak üzere 5 gruba ayrıldı. 1 ve 2. gruplardaki sıçanlara 7 gün boyunca gavajla astaksantin çözücüsü (zeytinyağı) verildi. 3, 4 ve 5. gruplardaki sıçanlara ise 7 gün boyunca gavajla zeytinyağında çözülmüş farklı dozlarda astaksantin verildi. 7. günün sonunda tüm gruplardaki sıçanlara bilateral olarak 45 dk böbrek iskemi sonrası 24 saat reperfüzyon yapıldı. Serum örneklerinden BUN ve Kreatinin seviyeleri ölçüldü. TAS-TOS düzeyleri ELİSA yöntemi ile ölçüldü. Böbrek dokusundan SOD ve MDA düzeyleri ölçüldü. Böbrek dokuları hematoksilen-eozin boyama metodu ile boyandı. Böbrek dokularında Beclin-1, LC3 ve p62 seviyeleri immünohistokimyasal yöntemle belirlendi. Bulgular: İ/R grubunun BUN ve kreatinin seviyelerinin sham grubuna göre artış gösterdiği, astaksantin uygulanan gruplarda ise anlamlı derecede düşük olduğu belirlendi (p<0.05). SOD ve TAS değerlerinin İ/R grubunda azaldığı, astaksantin uygulamasıyla arttığı belirlendi (p<0.05). MDA ve TOS değerlerinin İ/R grubunda arttığı ve astaksantin uygulamasıyla azaldığı belirlendi (p<0.05). İ/R grubunda tübüllerde nekrotik değişiklikler ve dilatasyon gözlendi. İ/R grubunda izlenen histopatolojik bulguların Atx 10 mg/kg+İ/R ve Atx 25 mg/kg+İ/R gruplarında belirgin derecede azaldığı tespit edildi (p<0.05). Beclin-1 ve LC3 immünreaktivitesi diğer gruplar ile karşılaştırıldığında en yüksek Atx 25 mg/kg+İ/R grubunda tespit edildi (p<0.05). p62 immünreaktivitesi ise en düşük Atx 25 mg/kg+İ/R grubunda tespit edildi (p<0.05). Sonuç: Çalışmamızda astaksantinin otofajinin indüksiyonu yoluyla dokuları hasara karşı koruduğu tespit edildi.
Aim: The aim of this study is to investigate effect of astaxanthin on autophagy in rats with renal ischemia reperfusion injury. Material and Method: In this study 40 male Sprague-dawley rats were used. The rats were divided into 5 groups; Group1 (Sham), Group2 (I/R), Group3 (Atx 5 mg/kg+I/R), Group4 (Atx 10 mg/kg+I/R) and Group 5 (Atx 25 mg/kg+I/R). The rats in groups 1 and 2 were given astaxanthin solvent (olive oil) by gavage for 7 days. The rats in groups 3, 4 and 5 were given different doses of astaxanthin dissolved in olive oil by gavage during 7 days. At the end of 7th day, rats in all groups were performed bilateral renal ischemia for 45 minutes then reperfusion for 24 hours. BUN and creatinine levels from serum samples were measured. TAS-TOS levels were measured by ELİSA method. SOD and MDA levels from renal tissue were measured. Kidney tissues were stained with Hematoxylin-eosin method. Beclin-1, LC3, p62 levels in renal tissues were detected by immunohistochemistry method. Results: The levels of BUN and creatinine in the I/R group were detected to increase relative to the sham group, while in the astaxanthin groups it was lower. (p<0.05). It was determined that the TAS and SOD values decreased in the I/R group and increased with astaxanthin administration (p<0.05). It was determined that MDA and TOS values increased in the I/R group and decreased with astaxanthin administration (p<0.05). Necrotic changes and dilatation were observed in the tubules in area in the I/R group. It was detected that the histopathological findings observed in the I/R group decreased significantly in the Atx 10 mg/kg+I/R and Atx 25 mg/kg+I/R groups (p<0.05). LC3 and Beclin-1 immunoreactivity were detected the highest in Atx 25 mg/kg+I/R group when compared another groups (p<0.05). p62 immunoreactivity was detected the lowest in the Atx 25 mg/kg+I/R group (p<0.05). Conclusion: In our study, it was determined that astaxanthin protects the tissues against damage via induction of autophagy
Aim: The aim of this study is to investigate effect of astaxanthin on autophagy in rats with renal ischemia reperfusion injury. Material and Method: In this study 40 male Sprague-dawley rats were used. The rats were divided into 5 groups; Group1 (Sham), Group2 (I/R), Group3 (Atx 5 mg/kg+I/R), Group4 (Atx 10 mg/kg+I/R) and Group 5 (Atx 25 mg/kg+I/R). The rats in groups 1 and 2 were given astaxanthin solvent (olive oil) by gavage for 7 days. The rats in groups 3, 4 and 5 were given different doses of astaxanthin dissolved in olive oil by gavage during 7 days. At the end of 7th day, rats in all groups were performed bilateral renal ischemia for 45 minutes then reperfusion for 24 hours. BUN and creatinine levels from serum samples were measured. TAS-TOS levels were measured by ELİSA method. SOD and MDA levels from renal tissue were measured. Kidney tissues were stained with Hematoxylin-eosin method. Beclin-1, LC3, p62 levels in renal tissues were detected by immunohistochemistry method. Results: The levels of BUN and creatinine in the I/R group were detected to increase relative to the sham group, while in the astaxanthin groups it was lower. (p<0.05). It was determined that the TAS and SOD values decreased in the I/R group and increased with astaxanthin administration (p<0.05). It was determined that MDA and TOS values increased in the I/R group and decreased with astaxanthin administration (p<0.05). Necrotic changes and dilatation were observed in the tubules in area in the I/R group. It was detected that the histopathological findings observed in the I/R group decreased significantly in the Atx 10 mg/kg+I/R and Atx 25 mg/kg+I/R groups (p<0.05). LC3 and Beclin-1 immunoreactivity were detected the highest in Atx 25 mg/kg+I/R group when compared another groups (p<0.05). p62 immunoreactivity was detected the lowest in the Atx 25 mg/kg+I/R group (p<0.05). Conclusion: In our study, it was determined that astaxanthin protects the tissues against damage via induction of autophagy
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Kısaoğlu, A. (2020). Böbrek iskemi-reperfüzyon modeli oluşturulan ratlarda astaksantin maddesinin otofaji üzerine etkisinin araştırılması, Yayınlanmış Yüksek Lisans Tezi, İnönü Üniversitesi.
