Effects of L-NAME and timolol on aqueous IL-1?, IL-6 IL-8, TNF-? and NO levels after Nd:YAG laser iridotomy in rabbits

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dc.authorwosidEvereklioğlu, Cem/A-5370-2018
dc.authorwosidcekmen, mustafa baki/G-6122-2011
dc.contributor.authorEr, H
dc.contributor.authorDoganay, S
dc.contributor.authorEvereklioglu, C
dc.contributor.authorCekmen, M
dc.contributor.authorDaglioglu, MC
dc.contributor.authorIsci, N
dc.date.accessioned2024-08-04T20:13:09Z
dc.date.available2024-08-04T20:13:09Z
dc.date.issued2002
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPURPOSE. Pro-inflammatory cytokines are produced by tissues and play a vital role in the host inflammatory response and uveitis. Nitric oxide (NO) can be produced in large amounts as a response to experimentally-induced uveitis or cytokines. In this study, we measured the levels of pro-inflammatory cytokines such as interleukin (IL)-1beta, IL-6, IL-8, tumor necrosis factor (TNF)-alpha and free-radical in aqueous humor after Nd:YAG laser iridotomy in rabbits, and investigated whether timolol maleate an anti-glaucoma drug, or a NO synthase inhibitor, NG-nitro-L-arginine methyl esther (L-NAME) had an inhibitory effect on these molecules, since L-NAME is a known anti-inflammatory agent in rabbits. METHODS. Bilateral experimental Nd:YAG laser iridotomy (power 7.5 mJ, mode single burst, aiming beam 4) was performed on 18 rabbits under general plus topical anesthesia. Aqueous humor samples were taken by clear corneal paracentesis preoperatively, and 1 and 24 h postoperatively. Six rabbits (12 eyes) were given bilateral topical timolol maleate 0.5% (Timoptic(R)) drop b.i.d (group 1), six rabbits (12 eyes) received bilateral 0.1 ml subconjuntival injections of L-NAME (150 mg/kg) (group 2), and six rabbits (12 eyes) were treated with topical balanced salt solution (BSS) b.i.d. (control). RESULTS. Preoperative cytokine and NO levels were comparable in the three groups, with no significant differences. In addition, there was no significant difference in baseline cytokine levels between the right and left eyes. In all groups, pre- and postoperative mean IL-1beta levels were below the:detection limit of the assay (< 5.0 pg/ml). In the control group, postoperative mean IL-6, IL-8 and NO levels were significantly higher after Nd: YAG laser iridotomy than before (for each, p < 0.01). Timolol and L-NAME both inhibited the rise in IL-8 and TNF-alpha levels. Timolol also inhibited the rise in IL-6 but not NO. L-NAME had an inhibitory effect against NO, but not IL-6. CONCLUSIONS. L-NAME has an inhibitory effect on IL-8, TNF-alpha and NO, but not on IL-6. Timolol had inhibitory effects on IL-6, IL-8 and TNF-alpha. but not on NO. These preliminary experimental results might help in assessing the effect of Nd: YAG laser iridotomy in aqueous humor, and to understand the inhibitory effects of timolol and L-NAME against these molecules.en_US
dc.identifier.doi10.1177/112067210201200405
dc.identifier.endpage286en_US
dc.identifier.issn1120-6721
dc.identifier.issn1724-6016
dc.identifier.issue4en_US
dc.identifier.pmid12219997en_US
dc.identifier.scopus2-s2.0-0036657383en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage281en_US
dc.identifier.urihttps://doi.org/10.1177/112067210201200405
dc.identifier.urihttps://hdl.handle.net/11616/93426
dc.identifier.volume12en_US
dc.identifier.wosWOS:000177771800005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofEuropean Journal of Ophthalmologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectaqueous humoren_US
dc.subjectcytokinesen_US
dc.subjectnitric oxideen_US
dc.subjectNd : YAG laseren_US
dc.subjectiridotomyen_US
dc.titleEffects of L-NAME and timolol on aqueous IL-1?, IL-6 IL-8, TNF-? and NO levels after Nd:YAG laser iridotomy in rabbitsen_US
dc.typeArticleen_US

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