Obezite oluşturulan sıçanlarda intraserebroventriküler MOTS-c infüzyonunun iştah mekanizması üzerine etkileri
Küçük Resim Yok
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Hipotalamusta ARC'den salgılanan nöropeptit ajanlar ve periferik dokudan salgılanan leptin ve ghrelin hormonlarıyla beslenme davranışını düzenlemektedir. MOTS-c 2015 yılında Lee ve ark. tarafından keşfedilmiş ve mtDNA'dan sentezlenen bir peptit olduğu bildirilmiştir. Bu çalışmanın amacı MOTS-c'nin besin alımı üzerine etkileri, NPY ve POMC nöropeptitleri ile leptin ve ghrelin hormon seviyelerinin belirlenmesidir. Materyal ve Metot: Çalışmada ortalama ağırlığı 30-50 gr olan Wistar Albino cinsi 40 adet erkek sıçan kullanıldı. 17 hafta boyunca YYDY ile ad libitum olarak beslenen sıçanlarda obezite oluşturularak hayvanlar 4 eşit gruba ayrıldı (n=10). Kontrol grubuna herhangi bir müdahalede bulunulmadı, sham kontrol grubuna ve deney gruplarına 14 gün boyunca günlük 120 µl (5 µl/sa) infüzyon sağlayan ozmotik mini pompalar lateral ventriküllere yerleştirilmiştir. Bu şekilde sham kontrol grubuna yBOS, deney gruplarına 10 µM ve 100 µM dozlarında MOTS-c infüzyonu sağlanmıştır. 14 günün sonunda hayvanlar dekapite edilerek beyin dokuları ve kan örnekleri toplandı. Kan örneklerinden ELISA yöntemiyle serum leptin ve ghrelin seviyeleri, hipotalamus dokularında RT-PCR yöntemiyle NPY, AgRP, POMC ve CART mRNA düzeyleri ve Western Blot yöntemiyle bu genlere ait protein miktarları belirlendi. Bulgular: Uygulanan 10 µM ve 100 µM MOTS-c miktarının besin alımını arttırırken (p<0.05) vücut ağırlığında istatistiksel olarak anlamlı düzeyde arttırmadığı (p>0.05); POMC ve AgRP peptid seviyelerine etki etmediği (p>0.05) gözlendi. NPY protein seviyesini arttırırken (p<0.05) CART seviyesini azalttığı belirlendi. Her iki dozun serum leptin seviyesini azaltırken ghrelin seviyesini arttırdığı görüldü (p<0.05). Sonuç: Bulgular MOTS-c'nin iştahı arttırdığını ancak vücut ağırlığında artışı engellediğini göstermektedir. Anahtar Kelimeler: MOTS-c, obezite, gıda alımı, vücut ağırlığı, beslenme davranışı
Aim: ARC expresses NPY, AgRP, POMC and CART peptides and these peptides regulate feeding behavior with leptin and ghrelin hormones. Mitochondrial derived peptide MOTS-c was explored by Lee et al in 2005. The aim of this study was investigate the effects of MOTS-c on food intake explained with detecting NPY, AgRP, POMC and CART neuropeptides associated with leptin and ghrelin hormones. Material and Method: In this study 30-50 g in weight 40 Wistar Albino male rats were used. Developed obesity on the rats by fed ad libitum with HFD for 17 weeks and evenly separated into 4 groups (n=10). Any operation was applied on the control group, osmotic mini pumps were implanted on lateral ventricules of sham and experimental groups and supplied 120 µl (5µl/sa) infusion volume for 14 days. ACSF infusion applied on sham group and 10 µM and 100 µM MOTS-c infusion applied on experimental groups. Throughout the experimental period, rats kept in individual cages, body weight and food consumptions were daily recorded. At the end of 14 days rats were decapitated and brain tissues and blood samples were collected. POMC, CART, NPY and AgRP mRNAs detected by RT-PCR, protein levels were detected by Western Blot method. Serum leptin and ghrelin levels were determined by ELISA method from collected blood samples. Results: Applied 10 µM and 100 µM MOTS-c doses increased food intake (p<0.05) but didn't cause significant change in body weights (p>0.05). Administration of MOTS-c didn't effect AgRP and POMC peptide levels (p>0.05). Also MOTS-c increased NPY protein levels while it decreased CART protein levels (p<0.05). In addition MOTS-c decreased serum leptin levels while it increased serum ghrelin levels (p<0.05). Conclusion: However MOTS-c increases apetite it is prevent weight gain. Keywords: MOTS-c, obesity, food intake, body weight, feeding behavior
Aim: ARC expresses NPY, AgRP, POMC and CART peptides and these peptides regulate feeding behavior with leptin and ghrelin hormones. Mitochondrial derived peptide MOTS-c was explored by Lee et al in 2005. The aim of this study was investigate the effects of MOTS-c on food intake explained with detecting NPY, AgRP, POMC and CART neuropeptides associated with leptin and ghrelin hormones. Material and Method: In this study 30-50 g in weight 40 Wistar Albino male rats were used. Developed obesity on the rats by fed ad libitum with HFD for 17 weeks and evenly separated into 4 groups (n=10). Any operation was applied on the control group, osmotic mini pumps were implanted on lateral ventricules of sham and experimental groups and supplied 120 µl (5µl/sa) infusion volume for 14 days. ACSF infusion applied on sham group and 10 µM and 100 µM MOTS-c infusion applied on experimental groups. Throughout the experimental period, rats kept in individual cages, body weight and food consumptions were daily recorded. At the end of 14 days rats were decapitated and brain tissues and blood samples were collected. POMC, CART, NPY and AgRP mRNAs detected by RT-PCR, protein levels were detected by Western Blot method. Serum leptin and ghrelin levels were determined by ELISA method from collected blood samples. Results: Applied 10 µM and 100 µM MOTS-c doses increased food intake (p<0.05) but didn't cause significant change in body weights (p>0.05). Administration of MOTS-c didn't effect AgRP and POMC peptide levels (p>0.05). Also MOTS-c increased NPY protein levels while it decreased CART protein levels (p<0.05). In addition MOTS-c decreased serum leptin levels while it increased serum ghrelin levels (p<0.05). Conclusion: However MOTS-c increases apetite it is prevent weight gain. Keywords: MOTS-c, obesity, food intake, body weight, feeding behavior
Açıklama
Anahtar Kelimeler
Fizyoloji, Physiology
