SIÇANLARDA PEMETREXED KAYNAKLI KARACİĞER HASARINA KARŞI CHRYSİN’İN KORUYUCU ETKİSİNİN İNCELENMESİ
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Dosyalar
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Çalışmamızın amacı pemetrexed’in karaciğer üzerindeki olası zararlı
etkilerini ve chrysin’in karaciğer toksisitesine karşı koruyucu rolünü araştırmaktır.
Materyal ve Metot: Çalışmada Sprague-Dawley cinsi 50 adet sıçan kullanıldı.
Grup I kontrol grubu olarak kullanıldı. Grup II’ye p.o. mısır yağı (chrysin çözücüsü)
(5mg/kg/gün, 28 gün boyunca), grup III’e i.p. PMTX (1mg/kg/hafta, 4 hafta boyunca),
grup IV’e p.o. chrysin (50 mg/kg/gün, 28 gün boyunca), grup V’e i.p. PMTX
(1mg/kg/hafta, 4 hafta boyunca) + chrysin (50 mg/kg/gün, 28 gün boyunca) uygulandı.
Tüm sıçanlar 28. günün sonunda kan ve karaciğer dokuları alındıktan sonra sakrifiye
edildi. Serum örneklerinden AST ve ALT seviyeleri ölçüldü. Karaciğer dokusundan
SOD, MDA, TAS ve TOS düzeyleri ölçüldü. Histolojik olarak hematoksilen-eozin,
immünohistokimyasal olarakta kaspaz-3 ve TNF-alfa’ya yönelik boyamalar yapıldı.
Bulgular: Grupların AST ve ALT düzeylerinin, PMTX grubunda diğer gruplara
göre anlamlı şekilde yüksek olduğu tespit edildi (p<0.005). MDA düzeyleri
karşılaştırıldığında PMTX grubunda, kontrol, sham ve CHR gruplarına göre anlamlı
düzeyde arttığı (p<0.05), SOD enzim aktivitesi karşılaştırıldığında ise PMTX grubunda
diğer gruplara göre anlamlı düzeyde azaldığı tespit edildi (p<0.05). Karaciğer
dokusunda TAS düzeyleri incelendiğinde, PMTX grubunda kontrol, sham ve CHR
gruplarına göre anlamlı derece düşük bulundu. TOS düzeyleri PMTX grubunda diğer
gruplara göre artmakla beraber istatistiksel olarak anlamlı değildi. Gruplar arasında
histopatolojik olarak anlamlı fark oluşmadı. Pemetrexed uygulamasının kaspaz-3 ve
TNF-α immünreaktivitesini artırdığı ve bu artışın, diğer gruplar ile karşılaştırıldığında
istatistiksel olarak anlamlı olduğu saptandı (p<0.01). PMTX+CHR grubunda hem
kaspaz-3 hem de TNF-α immünreaktivitesinde belirgin bir azalma izlendi (p<0.001).
Sonuç: Çalışmamızda chrysin’in pemetrexed kaynaklı karaciğer toksisitesinde
antioksidan etki yoluyla dokuları hasara karşı koruduğu tespit edildi.
Aim: The aim of our study is to investigate the possible harmful effects of pemetrexed on rat liver and the protective role of chrysin against it. Material and Method: In this study 50 male Spraque-Dawley rats were used. Group I was used a control group. Group II received a p.o. corn oil (chrysin solvent) (5 mg/kg/day for 28 days), group III received an i.p. PMTX (1 mg/kg/week for 4 weeks), group IV received a p.o. chrysin (50 mg/kg/day for 28 days), and group V received an i.p. PMTX (1 mg/kg/week for 4 weeks) + chrysin (50 mg/kg/day). After collecting blood and liver tissues, all rats were slaughtered at the conclusion of the 28th day. Serum samples were used to determine the levels of AST and ALT. The levels of SOD, MDA, TAS, and TOS in liver tissue were measured. Hematoxylin and eosin staining were used histologically, as well as caspase-3 and TNF-alpha immunohistochemistry. Results: AST and ALT levels of the groups, PMTX group were detected significantly increased when compared to other groups (p<0.005). When MDA levels were compared, the PMTX group increased significantly (p<0.005) compared to the control, sham, and CHR groups, whereas SOD enzyme activity was found to be significantly lower in the PMTX group compared to the other groups (p<0.005). TAS levels in liver tissue were examined to be considerably lower in the PMTX group than in the control, sham, and CHR groups (p<0.005). Although TOS levels in the PMTX group were higher than in the other groups, the difference was not statistically significant. There was no histopathologically significant difference between the groups. Pemetrexed treatment was found to enhance caspase-3 and TNF- immunoreactivity, which was statistically significant when compared to other groups (p<0.01). In the PMTX+CHR group, immunoreactivity to caspase-3 and TNF- was significantly reduced (p<0.001). Conclusion: In our study, it was determined that chrysin protects tissues against damage in pemetrexed-induced liver toxicity by acting as an antioxidant.
Aim: The aim of our study is to investigate the possible harmful effects of pemetrexed on rat liver and the protective role of chrysin against it. Material and Method: In this study 50 male Spraque-Dawley rats were used. Group I was used a control group. Group II received a p.o. corn oil (chrysin solvent) (5 mg/kg/day for 28 days), group III received an i.p. PMTX (1 mg/kg/week for 4 weeks), group IV received a p.o. chrysin (50 mg/kg/day for 28 days), and group V received an i.p. PMTX (1 mg/kg/week for 4 weeks) + chrysin (50 mg/kg/day). After collecting blood and liver tissues, all rats were slaughtered at the conclusion of the 28th day. Serum samples were used to determine the levels of AST and ALT. The levels of SOD, MDA, TAS, and TOS in liver tissue were measured. Hematoxylin and eosin staining were used histologically, as well as caspase-3 and TNF-alpha immunohistochemistry. Results: AST and ALT levels of the groups, PMTX group were detected significantly increased when compared to other groups (p<0.005). When MDA levels were compared, the PMTX group increased significantly (p<0.005) compared to the control, sham, and CHR groups, whereas SOD enzyme activity was found to be significantly lower in the PMTX group compared to the other groups (p<0.005). TAS levels in liver tissue were examined to be considerably lower in the PMTX group than in the control, sham, and CHR groups (p<0.005). Although TOS levels in the PMTX group were higher than in the other groups, the difference was not statistically significant. There was no histopathologically significant difference between the groups. Pemetrexed treatment was found to enhance caspase-3 and TNF- immunoreactivity, which was statistically significant when compared to other groups (p<0.01). In the PMTX+CHR group, immunoreactivity to caspase-3 and TNF- was significantly reduced (p<0.001). Conclusion: In our study, it was determined that chrysin protects tissues against damage in pemetrexed-induced liver toxicity by acting as an antioxidant.
Açıklama
Anahtar Kelimeler
pemetrexed, karaciğer toksisitesi, oksidatif stres
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Özdemir, B. (2022). Sıçanlarda Pemetrexed Kaynaklı Karaciğer Hasarına Karşı Chrysin’in Koruyucu Etkisinin İncelenmesi. Yüksek Lisans Tezi, İnönü Üniversitesi.
