Population Data and Internal Validation of the 21 Short Tandem Repeat Loci in Turkish Population

Basit Öğe Kaydı
dc.authoridOZTURK, Onur/0000-0003-3404-4135
dc.authoridCANPOLAT, ELIF/0000-0003-2500-3999
dc.authoridErkan, Itir/0000-0002-5902-1936
dc.authorwosidKaratas, Ömer/E-1071-2019
dc.authorwosidYonar, Fatma Cavus/H-8309-2018
dc.authorwosidOZTURK, Onur/HLH-4990-2023
dc.authorwosidERKAN, ITIR/I-4632-2016
dc.contributor.authorYukseloglu, Emel Hulya
dc.contributor.authorYonar, Fatma Cavus
dc.contributor.authorKaratas, Omer
dc.contributor.authorRayimoglu, Gulten
dc.contributor.authorAsicioglu, Faruk
dc.contributor.authorCanpolat, Elif
dc.contributor.authorOzturk, Onur
dc.date.accessioned2024-08-04T20:53:17Z
dc.date.available2024-08-04T20:53:17Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe purpose of this study was to identify paternity relations and increase the likelihood success in DNA identification of biological samples found in mass disasters and criminal events by using the 6-dye GlobalFilerTM amplification kit with 21 autosomal STR loci in our laboratory for validation purpose and routine use. In this regard, swab samples from different regions of Turkey (n=350) were collected and studied. The validation of the GlobalFilerTM Amplification Kit was carried out in accordance with the guidelines published by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our study was conducted to show the efficacy and performance of the kit for sensitivity, specificity, limit of detection, dynamic range, limit of quantification, stochastic threshold study, reproducibility and repeatability, mixture and contamination study parameters. Validation parameters and population genetics data were calculated by using Arlequin v 3.5.2.2 version. According to our results, SE33 (PM=0.014) locus showed the greatest power of discrimination and TPOX (PM=0.132) has the least power of discrimination power in Turkish population. Combined power of exclusion and combined power of discrimination were calculated as 99.99999963% and 99.99999999%, respectively. In addition, allelic frequencies from our study were compared to previously published population data of Iraq, Saud Arabia, China (Han), USA (Cauc), Iran, Afghan, Azerbaijan, Romania, and South Africa. The study revealed that there was a significant difference between Turkish and South Africa (0.1198) population and no difference was observed for Azerbaijan (0.0097) population.en_US
dc.description.sponsorshipIstanbul University-Cerrahpasa, Scientific Research Fund; [56104]en_US
dc.description.sponsorshipACKNOWLEDGMENTS This study was supported by Istanbul University-Cerrahpasa, Scientific Research Fund (Project No: 56104) .en_US
dc.identifier.doi10.17582/journal.pjz/20210803110808
dc.identifier.endpage512en_US
dc.identifier.issn0030-9923
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85144924465en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage501en_US
dc.identifier.urihttps://doi.org/10.17582/journal.pjz/20210803110808
dc.identifier.urihttps://hdl.handle.net/11616/101083
dc.identifier.volume55en_US
dc.identifier.wosWOS:000905139500001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherZoological Soc Pakistanen_US
dc.relation.ispartofPakistan Journal of Zoologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAllele frequencyen_US
dc.subjectGlobalFilerTM kiten_US
dc.subjectInternal validationen_US
dc.subjectPopulationen_US
dc.subjectSTR markersen_US
dc.titlePopulation Data and Internal Validation of the 21 Short Tandem Repeat Loci in Turkish Populationen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu