Characterization of the BETA1 gene, which might play a role in Beta vulgaris subsp maritima salt tolerance
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technological Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Salinity stress has a negative impact on plant growth, which affects homeostasis and productivity. The uptake of nonessential salt ions changes the osmotic balance of the cell and causes dehydration. Higher plants develop salt tolerance mechanisms to avoid dehydration. Sea beet (Beta vulgaris subsp. maritima) is a halophytic ancestor of cultivated sugar beet that displays salt stress tolerance. In this study, we screened a B. vulgaris subsp. maritima cDNA library in Saccharomyces cerevisiae strain Ab1 1c (ena1 triangle, nha 1/4 triangle, nhx1 triangle), which is deficient in sodium transport, to find sodium-detoxifying genes. We identified a cDNA construct, named BETA 1, providing salt tolerance to yeast cells. This gene had no previously described function. Intracellular sodium measurements demonstrated no significant differences between yeast cells expressing BETA1 or a sham vector, suggesting that sodium was not effluxed in BEZA1-expressing cells. Transcriptionally, BETA1 mRNA levels were induced immediately in leaves and later in the root system in response to the salt stress. Our results suggest that the BETA1 gene is part of the salt tolerance network in B. vulgaris subsp. maritima.
Açıklama
Anahtar Kelimeler
Salt stress, yeast, BETA1, Beta vulgaris subsp maritima
Kaynak
Turkish Journal of Botany
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
41
Sayı
6
