Salivary Alpha Amylase Enzyme as a Stress Parameter: Establishment and Comparison of Laboratory Methods

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dc.contributor.authorBarutcu, Ozlem
dc.contributor.authorYildiz, Sedat
dc.date.accessioned2024-08-04T21:01:04Z
dc.date.available2024-08-04T21:01:04Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAim: Salivary alpha ( alpha )-amylase enzyme is a biomarker used to measure sympathetic nervous system activity. This study aimed to establish three existing methods for measuring salivary alpha- amylase enzyme activity in the laboratory and to compare these methods in terms of their usability. Materials and Methods: alpha- amylase enzyme activity can be measured in ready-made kits by three methods: Starch-Iodine, 2-chloro-4-nitrophenyl maltotrioside (CNPG3) and dinitrosalicylic acid (DNS) methods. In this context, standard curves were created in the laboratory for manual study of these methods and their advantages and disadvantages were presented. The usability of these methods was tested. Results: The starch-iodine and CNPG3 methods were successfully established. However, although a standard curve was successfully established for the DNS method, this assay was not suitable for the studies as the samples were not readable and had many disadvantages. The test sensitivities and working ranges were appropriate for the starch-iodine and CNPG3 tests, requiring 4,000-fold dilution for the starch-iodine test and 5-fold dilution for the CNPG3 test. A weak but statistically significant positive correlation was observed between the two tests (R2=0.048 for linear regression; p<0.05, R2=0.106 for quadratic regression; p<0.01). Conclusion: The CNPG3 and starch-iodine methods were feasible, cost-effective, accessible, and time-efficient. The starch-iodine method is a cheaper but the CNPG3 method is also a practical test with fewer steps. In this respect, it has been decided that the CNPG3 method is the most effective method in studies based on salivary alpha- amylase enzyme method.en_US
dc.description.sponsorshipIdot;nonu University Scientific Research Projects [2015/82]en_US
dc.description.sponsorshipThis study was supported by & Idot;nonu University Scientific Research Projects with project number: 2015/82.en_US
dc.identifier.doi10.4274/nkmj.galenos.2024.02418
dc.identifier.endpage127en_US
dc.identifier.issn2587-0262
dc.identifier.issue2en_US
dc.identifier.startpage122en_US
dc.identifier.urihttps://doi.org/10.4274/nkmj.galenos.2024.02418
dc.identifier.urihttps://hdl.handle.net/11616/104067
dc.identifier.volume12en_US
dc.identifier.wosWOS:001266644800007en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofNamik Kemal Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectalpha- Amylase enzymeen_US
dc.subjectCNPG3en_US
dc.subjectDNSen_US
dc.subjectstarch-iodineen_US
dc.subjectsalivaen_US
dc.titleSalivary Alpha Amylase Enzyme as a Stress Parameter: Establishment and Comparison of Laboratory Methodsen_US
dc.typeArticleen_US

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