Evaluation of the effects of adipose-derived mesenchymal stem cells on intraperitoneal adhesions
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Aves, buyukdere cad 105-9, mecıdıyekoy, sıslı, ıstanbul 34394, turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objectives: The goal was to examine the efficiency of local implementation of adipose-derived mesenchymal stem cells, which have an anti-inflammatory effect, in preventing the intra-abdominal adhesions in rats.
Material and Methods: Twenty-one Wistar albino rats were randomly divided into 3 groups, 7 rats in each: Group 1 was defined as the control group, Group 2 as the sham group, and Group 3 as the adipose-derived mesenchymal stem cell group. A 6 cm mid-abdomen incision in the all the rats was performed. The cecum serosa and sub-serosa were injured by rubbing with a gauze. No agent was applied intraperitoneally for the rats in Group 1; 1.5 mL saline and 2x106/ kg allojenic adipose-derived mesenchymal stem cells in the 1.5 mL saline were injected into peritoneum of rats in Groups 2 and 3, respectively. Laparotomy was performed on the 14th day. Adhesion scores, histopathological examination, E-cadherin expression, and the tissue hydroxyproline level were evaluated.
Results: The general adhesion score and collagen deposition in Group 3 were found to be significantly higher than in Groups 1 and 2 (p=0.003 and p=0.009, respectively). In the inflammatory cell comparison, a significant decrease was found in Group 3 in proportion to Groups 1 and 2 (p=0.001, p=0.005, respectively). The E-cadherin levels were found to be higher in Group 3 (p=0.003).
Conclusion: Severe adhesion was observed in the adipose-derived mesenchymal stem cells group. Collagen intensity and E-Cadherin expression also increased in the adipose-derived mesenchymal stem cells group. The anti-inflammatory effect was also seen in the adipose-derived mesenchymal stem cells group.
Açıklama
Anahtar Kelimeler
Perıtoneal adhesıons, postoperatıve adhesıons, mesothelıal cells, preventıon, model, ınjury, rats, ınflammatıon, mechanısms, transıtıon
Kaynak
Turkısh journal of surgery
WoS Q Değeri
Scopus Q Değeri
Cilt
34
Sayı
3
Künye
Uysal, E. Dokur, M. Kirdak, T. Kurt, A . Karadag, M. (2018). Evaluation of the effects of adipose-derived mesenchymal stem cells on intraperitoneal adhesions. Cilt:34. Sayı:3. 184-190 ss.
