Evaluation of the effects of adipose-derived mesenchymal stem cells on intraperitoneal adhesions
| dc.contributor.author | Uysal, Erdal | |
| dc.contributor.author | Dokur, Mehmet | |
| dc.contributor.author | Kirdak, Turkay | |
| dc.contributor.author | Kurt, Akif | |
| dc.contributor.author | Karadag, Mehmet | |
| dc.date.accessioned | 2019-07-11T11:43:59Z | |
| dc.date.available | 2019-07-11T11:43:59Z | |
| dc.date.issued | 2018 | |
| dc.department | İnönü Üniversitesi | en_US |
| dc.description.abstract | Objectives: The goal was to examine the efficiency of local implementation of adipose-derived mesenchymal stem cells, which have an anti-inflammatory effect, in preventing the intra-abdominal adhesions in rats. Material and Methods: Twenty-one Wistar albino rats were randomly divided into 3 groups, 7 rats in each: Group 1 was defined as the control group, Group 2 as the sham group, and Group 3 as the adipose-derived mesenchymal stem cell group. A 6 cm mid-abdomen incision in the all the rats was performed. The cecum serosa and sub-serosa were injured by rubbing with a gauze. No agent was applied intraperitoneally for the rats in Group 1; 1.5 mL saline and 2x106/ kg allojenic adipose-derived mesenchymal stem cells in the 1.5 mL saline were injected into peritoneum of rats in Groups 2 and 3, respectively. Laparotomy was performed on the 14th day. Adhesion scores, histopathological examination, E-cadherin expression, and the tissue hydroxyproline level were evaluated. Results: The general adhesion score and collagen deposition in Group 3 were found to be significantly higher than in Groups 1 and 2 (p=0.003 and p=0.009, respectively). In the inflammatory cell comparison, a significant decrease was found in Group 3 in proportion to Groups 1 and 2 (p=0.001, p=0.005, respectively). The E-cadherin levels were found to be higher in Group 3 (p=0.003). Conclusion: Severe adhesion was observed in the adipose-derived mesenchymal stem cells group. Collagen intensity and E-Cadherin expression also increased in the adipose-derived mesenchymal stem cells group. The anti-inflammatory effect was also seen in the adipose-derived mesenchymal stem cells group. | en_US |
| dc.identifier.citation | Uysal, E. Dokur, M. Kirdak, T. Kurt, A . Karadag, M. (2018). Evaluation of the effects of adipose-derived mesenchymal stem cells on intraperitoneal adhesions. Cilt:34. Sayı:3. 184-190 ss. | en_US |
| dc.identifier.doi | 10.5152/turkjsurg.2017.3860 | en_US |
| dc.identifier.endpage | 190 | en_US |
| dc.identifier.issue | 3 | en_US |
| dc.identifier.startpage | 184 | en_US |
| dc.identifier.uri | https://hdl.handle.net/11616/12535 | |
| dc.identifier.volume | 34 | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Aves, buyukdere cad 105-9, mecıdıyekoy, sıslı, ıstanbul 34394, turkey | en_US |
| dc.relation.ispartof | Turkısh journal of surgery | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Perıtoneal adhesıons | en_US |
| dc.subject | postoperatıve adhesıons | en_US |
| dc.subject | mesothelıal cells | en_US |
| dc.subject | preventıon | en_US |
| dc.subject | model | en_US |
| dc.subject | ınjury | en_US |
| dc.subject | rats | en_US |
| dc.subject | ınflammatıon | en_US |
| dc.subject | mechanısms | en_US |
| dc.subject | transıtıon | en_US |
| dc.title | Evaluation of the effects of adipose-derived mesenchymal stem cells on intraperitoneal adhesions | en_US |
| dc.type | Article | en_US |
