Tailor-made shape memory stents for therapeutic enzymes: A novel approach to enhance enzyme performance

Basit Öğe Kaydı
dc.authoridAteş, Burhan/0000-0001-6080-229X
dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authorwosidAteş, Burhan/AAA-3730-2021
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.contributor.authorUlu, Ahmet
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:50:23Z
dc.date.available2024-08-04T20:50:23Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractHerein, our suggestion is to immobilize enzymes in-situ on absorbable shape-memory stents instead of injecting therapeutic enzymes into the blood. Chitosan (CHI)-based stents were tailored as novel support and the enzyme-immobilizing ability was elucidated using L-asparaginase (L-ASNase). For developing shape-memory stents, CHI-glycerol (GLY) solution was prepared and further blended with different ratios of polyethylene glycol (PEG), and polyvinyl alcohol (PVA). Afterward, the blends were modified by ionic crosslinking with sodium tripolyphosphate to obtain a shape-memory character. L-ASNase was included in the blends by using in-situ method before ionic crosslinking. The prepared stents, with or without L-ASNase, were comprehensively characterized by using several techniques. Collectively, immobilized L-ASNase exhibited much better performance in immobilization parameters than free one, thanks to its improved stability and reusability. For instance, CHI/GLY/PEG-3@L-ASNase retained about 70% of the initial activity after storage at 30 degrees C for 2 weeks, whereas the free form lost half of its initial activity. Besides, it retained 73.4% residual activity after 15 consecutive cycles. Most importantly, stent formulations exhibited similar to 60% activity in the bioreactor system after 4 weeks of incubation. Given the above results, shape-memory stents can be a promising candidate as a new platform for immobilization, especially in the blood circulation system.en_US
dc.description.sponsorshipInonu University Sci-entific Research Projects Unit [FDK751, 2019557114]en_US
dc.description.sponsorshipThis work was financially supported by The Inonu University Sci-entific Research Projects Unit [Grant number: FDK751] and derived as a part of Ph.D. thesis presented by Ahmet Ulu (2019557114) .en_US
dc.identifier.doi10.1016/j.ijbiomac.2021.07.003
dc.identifier.endpage982en_US
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid34237367en_US
dc.identifier.scopus2-s2.0-85109148655en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage966en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2021.07.003
dc.identifier.urihttps://hdl.handle.net/11616/100024
dc.identifier.volume185en_US
dc.identifier.wosWOS:000680062600009en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectShape-memory stentsen_US
dc.subjectL-asparaginaseen_US
dc.subjectBiocatalystsen_US
dc.subjectEnhanced stabilityen_US
dc.titleTailor-made shape memory stents for therapeutic enzymes: A novel approach to enhance enzyme performanceen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu