Species distribution, antifungal susceptibility and clonal relatedness of Candida isolates from patients in neonatal and pediatric intensive care units at a medical center in Turkey

Basit Öğe Kaydı
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridDURMAZ, RIZA/0000-0001-6561-778X
dc.authoridaktas, elif/0000-0003-3087-5425
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.authorwosidDURMAZ, Rıza/HJH-4918-2023
dc.authorwosidaktas, elif/ACL-9792-2022
dc.contributor.authorKuzucu, Cigdem
dc.contributor.authorDurmaz, Riza
dc.contributor.authorOtlu, Baris
dc.contributor.authorAktas, Elif
dc.contributor.authorGulcan, Hande
dc.contributor.authorCizmeci, Zaynep
dc.date.accessioned2024-08-04T20:30:59Z
dc.date.available2024-08-04T20:30:59Z
dc.date.issued2008
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe aim of this study was to assess species distribution, antifungal susceptibility and clonal relationships among Candida strains isolated from a group of pediatric/neonatal intensive care (PICU/NICU) patients that had a very high mortality rate (76%). The cases of 21 patients (19 with candidemia, 2 with Candida meningitides) treated over a 1-year period in a Turkish hospital PICU and NICU were retrospectively analyzed. Twenty-eight Candida isolates were detected from blood (20), cerebrospinal fluid (CSF) (2) and other specimens (6). Candida species were identified using the API ID 32C System. Susceptibility testing was done (all 28 isolates) for amphotericin B, fluconazole and itraconazole using the broth microdilution method. Arbitrarily primed polymerase chain reaction (AP-PCR) was used for molecular typing of the 3 most common ones; C. albicans (15), C. parapsilosis (6), and C. pelliculosa (4). Electrophoretic karyotyping (EK) was done to check clonal identity obtained by AP-PCR. Of the 20 blood isolates, 8 (40%) were C. albicans, 12 (60%) were non-albicans Candida, and one of the 2 CSF isolates was C. albicans. The overall species distribution was as follows: 15 C. albicans isolates, 6 C. parapsilosis isolates, 4 C. pelliculosa isolates, 2 C. famata isolates and I C. tropicalis isolate. Amphotericin B had the best antifungal activity with a MIC90 of 0.125 mu g/ml, and the rates of susceptibility to fluconazole and itraconazole were 93% and 82%, respectively AP-PCR revealed 11 genotypes (4 were identical pairs, 7 were distinct) among the 15 C albicans isolates, 2 genotypes (5 were classified in the same type) among the 6 C. parapsilosis isolates, and 4 separate genotypes for the 4 C. pelliculosa isolates. Karyotyping results correlated well with the AP-PCR findings. As indicated in the previous research, our results confirmed that non-albicans Candida species have become more frequently causative agents for invasive fungal infections in the ICU. Transmission of C. albicans and C pelliculosa was relatively low, but transmission of C. parapsilosis was high, suggesting that more effective control and very strict treatment protocols are needed for patients having high mortality and invasive fungal infection in ICU.en_US
dc.description.sponsorshipInonu University Research Fund, Malatya, Turkey [2003142]en_US
dc.description.sponsorshipThis study was supported by Grant 2003142 from the Inonu University Research Fund, Malatya, Turkeyen_US
dc.identifier.endpage408en_US
dc.identifier.issn1121-7138
dc.identifier.issue3en_US
dc.identifier.pmid18843896en_US
dc.identifier.scopus2-s2.0-52749091267en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage401en_US
dc.identifier.urihttps://hdl.handle.net/11616/94654
dc.identifier.volume31en_US
dc.identifier.wosWOS:000259559800014en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherEdizioni Int Srlen_US
dc.relation.ispartofNew Microbiologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbloodstream infectionsen_US
dc.subjectCandida spp.en_US
dc.subjectneonatal intensive care uniten_US
dc.subjectarbitrarily primed polymerase chain reactionen_US
dc.subjectelectrophoretic karyotypingen_US
dc.subjectantifungal susceptibility testingen_US
dc.titleSpecies distribution, antifungal susceptibility and clonal relatedness of Candida isolates from patients in neonatal and pediatric intensive care units at a medical center in Turkeyen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu