tRNA Wobble Base Modifications and Boric Acid Resistance in Yeast: Boron-Resistant Deletion Mutants Induce the General Amino Acid Control Mechanism and Activate Boron Efflux

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dc.authorscopusid36624357200
dc.authorscopusid57217064965
dc.authorscopusid57196914953
dc.contributor.authorUluisik I.
dc.contributor.author? Karakaya H.
dc.contributor.authorKoc A.
dc.date.accessioned2024-08-04T19:59:12Z
dc.date.available2024-08-04T19:59:12Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBoric acid is essential for plants and has many vital roles in animals and microorganisms. However, its high doses are toxic to all organisms. We previously screened yeast deletion collections to identify boric acid-resistant and susceptible mutants to identify genes that play a role in boron tolerance. Here, we analyzed boron resistant mutants (elpl?, elp3?, elp6?, ncs2?, ncs6? and ktil2?) for their abilities to modulate the general amino acid control system (GAAC) and to induce boron efflux pump ATR1. The mutants analyzed in this study lack the genes that play roles in tRNA Wobble base modifications. We found that all of the boron resistant mutants activated Gcn4-dependent reporter gene activity and increased the transcript level of the ATR1 gene. Additionally, boron resistant cells accumulated less boric acid in their cytoplasm compared to the wild type cells upon boron exposure. Thus, our findings suggested that loss of wobble base modifications in tRNA leads to GAAC activation and ATR1 induction, which in turn reduced intracellular boron levels and caused boron resistance.en_US
dc.identifier.doi10.31857/S0026898420030180
dc.identifier.endpage456en_US
dc.identifier.issn0026-8984
dc.identifier.issue3en_US
dc.identifier.pmid32492007en_US
dc.identifier.scopus2-s2.0-85086008666en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage450en_US
dc.identifier.urihttps://doi.org/10.31857/S0026898420030180
dc.identifier.urihttps://hdl.handle.net/11616/90448
dc.identifier.volume54en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoruen_US
dc.publisherNLM (Medline)en_US
dc.relation.ispartofMolekuliarnaia biologiiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectboric aciden_US
dc.subjectboronen_US
dc.subjectboron resistanceen_US
dc.subjectelongator complexen_US
dc.subjectgeneral amino acid controlen_US
dc.subjecttRNAen_US
dc.subjectWobble baseen_US
dc.subjectyeasten_US
dc.titletRNA Wobble Base Modifications and Boric Acid Resistance in Yeast: Boron-Resistant Deletion Mutants Induce the General Amino Acid Control Mechanism and Activate Boron Effluxen_US
dc.typeArticleen_US

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