Isolation and identification of Mycobacterium tuberculosis from the urine samples by conventional and molecular methods

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dc.authoridbayram, gul/0000-0002-4154-8596
dc.authoridDURMAZ, RIZA/0000-0001-6561-778X
dc.authorwosidbayram, gul/JAC-9799-2023
dc.authorwosidserin, mehmet Sami/KCK-8718-2024
dc.authorwosidDURMAZ, Rıza/HJH-4918-2023
dc.authorwosidDirekel, Sahin/ABG-1575-2020
dc.authorwosidDoruk, Erdal/AIE-9217-2022
dc.authorwosidSerin, Mehmet Sami/G-1572-2015
dc.contributor.authorAslan, Gonul
dc.contributor.authorDoruk, Erdal
dc.contributor.authorEmekdas, Gurol
dc.contributor.authorSerin, M. Sami
dc.contributor.authorDirekel, Sahin
dc.contributor.authorBayram, Gul
dc.contributor.authorDurmaz, Riza
dc.date.accessioned2024-08-04T20:30:39Z
dc.date.available2024-08-04T20:30:39Z
dc.date.issued2007
dc.departmentİnönü Üniversitesien_US
dc.description.abstractGenitourinary tuberculosis presents a challenge in diagnosis and treatment due to variations in clinical and radiological signs, insufficient patient history and difficulty in the isolation of the bacilli. The aim of this study was to isolate and identify Mycobacterium tuberculosis from the urine samples obtained from patients with suspected urinary tuberculosis admitted to our hospital by using Ehrlich-Ziehl-Neelsen (EZN), culture and polymerase chain reaction-restriction analysis (PCR-RFLP) methods. A total of 1004 urine samples collected from 437 patients who were admitted to our hospital between January 2004- July 2006, were inoculated on Lowenstein-Jensen (LJ) and/or BACTEC 12B (Becton Dickinson, USA) after decontamination and, direct preparations stained with EZN method were evaluated microscopically. M.tuberculosis complex (MTC) and mycobacteria other than tuberculosis (MOTT) were differentiated by nitro-alpha-acetylamino-beta-hydroxypropiophenone (NAP) test and the susceptibility testing for the MTC strains to primary antituberculosis drugs were performed by BACTEC 460 TB (Becton Dickinson, USA) system. PCR-RFLP method was performed for the identification of Mycobacterium spp. Twenty-two (5%) patients have yielded positive results by at least one of the conventional methods (EZN, LJ and/or BACTEC). Fifteen samples were positive for acido-resistant bacilli (ARB) by EZN method, and 17 samples were positive for mycobacterial growth in the cultures. Ten of 22 patients were found positive by both of the methods, while seven were culture positive but ARB negative and five were culture negative but ARB positive. These five patients received BCG treatment because of the presence of bladder tumor. Twelve (70.5%) of 17 strains isolated from culture were identified as MTC, while five (29.4%) were identified as M.fortuitum. Of 12 MTC isolates, eight (66.7%) were found susceptible to all of the antituberculosis agents, while one was found resistant to isoniazide (INH) and ethambutole (ETB), one was resistant to INH and rifampicin (RIF), and two were resistant to only INK It is concluded that, in order to identify mycobacteria and to perform antituberculous susceptibility tests, cultivation of mycobacteria is a prerequisite.en_US
dc.identifier.endpage192en_US
dc.identifier.issn0374-9096
dc.identifier.issue2en_US
dc.identifier.pmid17682704en_US
dc.identifier.scopus2-s2.0-38549121489en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage185en_US
dc.identifier.urihttps://hdl.handle.net/11616/94440
dc.identifier.volume41en_US
dc.identifier.wosWOS:000246913000003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjecturinary tuberculosisen_US
dc.subjectEhrlich Ziehl Neelsenen_US
dc.subjectcultureen_US
dc.subjectmolecular diagnosisen_US
dc.titleIsolation and identification of Mycobacterium tuberculosis from the urine samples by conventional and molecular methodsen_US
dc.typeArticleen_US

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