Gene expression profiles of mitochondria-endoplasmic reticulum tethering in human gingival fibroblasts in response to periodontal pathogens

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dc.authoridAral, Kubra/0000-0003-4798-4548
dc.authorwosidAral, Kubra/R-3039-2017
dc.contributor.authorAral, Kubra
dc.contributor.authorMilward, Michael R.
dc.contributor.authorCooper, Paul R.
dc.date.accessioned2024-08-04T20:50:16Z
dc.date.available2024-08-04T20:50:16Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjective: The current study aimed to elucidate the potential involvement of mitochondria-endoplasmic reticulum contact genes in the pathogenesis of periodontal disease by monitoring levels of contact associated genes including Mitofusion 1 (MFN1) and MFN2, inositol 1,4,5-trisphosphate receptor (IP3R), chaperone glucoseregulated protein 75 (GRP75), sigma non-opioid intracellular receptor 1 (SIGMAR1) and phosphate and tensin homolog induced putative kinase 1 (PINK1) in human gingival fibroblasts in response to periodontal pathogens Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis) in vitro. Design: Primary human gingival fibroblasts were exposed to live cultures of P. gingivalis (W83; ATCC BAA-308) and F. nucleatum (subsp. Polymorphum; ATCC 10953) alone or in combination for 4 h at a 50 or 200 multiplicity of infection. Escherichia coli lipopolysaccharide (10 mu g/mL) exposure was used as a positive control. Gene expression levels of contact genes (MFN1, MFN2, IP3R, GRP75, SIGMAR1 and PINK1) as well as a proinflammatory cytokine, Tumor necrosis factor-alpha (TNF-alpha), and the apoptosis associated gene, Immediate early response 3 (IER3), were evaluated by reverse transcription polymerase chain reaction analysis. Results: MFN1, GRP75, IP3R and PINK1 were significantly upregulated by P. gingivalis with or without F. nucleatum. Only P. gingivalis with F. nucleatum caused a significant upregulation of SIGMAR1. TNF-alpha and IER3 gene expression positively correlated with the contact-associated gene expression changes. Conclusion: F. nucleatum and P. gingivalis alone or in combination may differentially dysregulate the gene expression levels of contact-associated genes in human gingival fibroblasts. These host-microbiome interactions may mechanistically be important in the pathogenesis of periodontal disease.en_US
dc.description.sponsorshipSchool of Dentistry, University of Birmingham; Scientific and Technological Research Council of Turkey (TUBITAK), Ankara, Turkeyen_US
dc.description.sponsorshipThis study was financially supported by the School of Dentistry, University of Birmingham. The corresponding author of this study (K. Aral) was supported with a scholarship by the Scientific and Technological Research Council of Turkey (TUBITAK), Ankara, Turkey.en_US
dc.identifier.doi10.1016/j.archoralbio.2021.105173
dc.identifier.issn0003-9969
dc.identifier.issn1879-1506
dc.identifier.pmid34058723en_US
dc.identifier.scopus2-s2.0-85107114841en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.archoralbio.2021.105173
dc.identifier.urihttps://hdl.handle.net/11616/99965
dc.identifier.volume128en_US
dc.identifier.wosWOS:000659789300010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofArchives of Oral Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPeriodontal diseaseen_US
dc.subjectEndoplasmic reticulumen_US
dc.subjectPorphyromonas gingivalisen_US
dc.subjectFusobacterium nucleatumen_US
dc.subjectMitochondriaen_US
dc.subjectGingival fibroblastsen_US
dc.titleGene expression profiles of mitochondria-endoplasmic reticulum tethering in human gingival fibroblasts in response to periodontal pathogensen_US
dc.typeArticleen_US

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