Investigation of Carbapenemases in Carbapenem-Resistant Escherichia coli and Klebsiella pneumoniae Strains Isolated in 2014 in Turkey

Basit Öğe Kaydı
dc.authoridYILMAZ, YAKUT AKYON/0000-0002-0919-5508
dc.authorid, yasemin/0000-0002-9078-9900
dc.authoridPARLAK, Mehmet/0000-0001-6030-2244
dc.authoridAy, selma/0000-0002-7640-6635
dc.authoridÇöplü, Nilay/0000-0003-1956-1417
dc.authoridOzhak, Betil/0000-0001-5224-1824
dc.authoridAKYON YILMAZ, YAKUT/0000-0002-0919-5508
dc.authorwosidYILMAZ, YAKUT AKYON/I-8298-2013
dc.authorwosidAktas, Zerrin/L-5381-2016
dc.authorwosidKILIÇ, HÜSEYİN/ABE-3397-2020
dc.authorwosid, yasemin/AAH-5440-2020
dc.authorwosidKilic, Abdullah/AAI-2792-2021
dc.authorwosidPARLAK, Mehmet/A-8579-2018
dc.authorwosidBayramoglu, Gulcin/AAR-2120-2020
dc.contributor.authorCakar, Asli
dc.contributor.authorAkyon, Yakut
dc.contributor.authorGur, Deniz
dc.contributor.authorKaratuna, Onur
dc.contributor.authorOgunc, Dilara
dc.contributor.authorBaysan, Betil Ozhak
dc.contributor.authorCoplu, Nilay
dc.date.accessioned2024-08-04T20:42:31Z
dc.date.available2024-08-04T20:42:31Z
dc.date.issued2016
dc.departmentİnönü Üniversitesien_US
dc.description.abstractCarbapenems are the choice of treatment in infections caused by multidrug resistant Enterobacteriaceae. In recent years carbapenem-resistant Enterobacteriaceae isolates due to carbapenemases have been increasingly reported worldwide. Multicenter studies on carbapenemases are scarce in Turkey. The aim of this study was to determine the distribution of carbapenemases from different parts of Turkey as a part of the European Survey of Carbapenemase Producing Enterobacteriaceae (EuSCAPE) project. Beginning in November 2013, carbapenem-resistant isolates resistant to at least one of the agents, namely imipenem, meropenem, and ertapenem were sent to the coordinating center. Minimum inhibitory concentrations for these carbapenems were determined by microdilution tests following EUCAST guidelines. Production of carbapenemase was confirmed by combination disk synergy tests. Types of carbapenemases were investigated using specific primers for VIM, IMP; NDM, KPC and OXA-48 genes by multiplex polymerase chain reaction. In a six month period, 155 suspected carbapenemase-positive isolates were sent to the coordinating center of which 21 (13.5%) were E.coli and 134 (86.5%) were K.pneumoniae. Nineteen (90.5%) strains among E.coli and 124 (92.5%) strains among K.pneumoniae were shown to harbour at least one carbapenemase gene by molecular tests, with a total of 92.3% (143/155). Carbapenemases were determined as a single enzyme in 136 strains (OXA-48: 84.6%; NDM: 6.3%; VIM: 2.8%; IMP: 1.4%) and as a combination in seven isolates (OXA-48 + NDM: 2.1%; OXA-48 + VIM: 2.1%; VIM + NDM: 0.7%). KPC was not detected in any of the isolates. According to the microdilution test results, resistance to imipenem, meropenem and ertapenem in OXA-48 isolates were 59.5%, 52.9% and 100%, respectively. The combination disk synergy test was 100% compatible with the molecular test results. As most of the OXA-48 producing isolates were susceptible to meropenem but all were resistant to ertapenem, ertapenem seems to be the most sensitive agent in screening carbapenemases in areas where OXA-48 is prevalent and phenotypic combination tests can be useful in centers where molecular tests are not available.en_US
dc.identifier.doi10.5578/mb.10695
dc.identifier.endpage33en_US
dc.identifier.issn0374-9096
dc.identifier.issue1en_US
dc.identifier.pmid27058326en_US
dc.identifier.scopus2-s2.0-84978208256en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage21en_US
dc.identifier.urihttps://doi.org/10.5578/mb.10695
dc.identifier.urihttps://hdl.handle.net/11616/97408
dc.identifier.volume50en_US
dc.identifier.wosWOS:000371499500003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCarbapenemaseen_US
dc.subjectEnterobacteriaceaeen_US
dc.subjectOXA-48en_US
dc.subjectmetallo-beta-lactamaseen_US
dc.subjectTurkeyen_US
dc.titleInvestigation of Carbapenemases in Carbapenem-Resistant Escherichia coli and Klebsiella pneumoniae Strains Isolated in 2014 in Turkeyen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu