Folic acid decoration of mesoporous silica nanoparticles to increase cellular uptake and cytotoxic activity of doxorubicin in human breast cancer cells

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dc.authoridEsendagli, Gunes/0000-0003-4865-2377
dc.authoridŞahin, Adem/0000-0002-3996-2931
dc.authoridTonbul, Hayrettin/0000-0001-5510-8973
dc.authoridUltav, Gozde/0000-0001-5582-3766
dc.authoridTavukcuoglu, Ece/0000-0003-3344-468X
dc.authorwosidEsendagli, Gunes/Q-5136-2019
dc.authorwosidŞahin, Adem/IYT-0077-2023
dc.authorwosidTonbul, Hayrettin/AAR-6961-2020
dc.authorwosidUltav, Gözde/JPK-6247-2023
dc.contributor.authorTonbul, Hayrettin
dc.contributor.authorSahin, Adem
dc.contributor.authorTavukcuoglu, Ece
dc.contributor.authorUltav, Gozde
dc.contributor.authorAkbas, Sedenay
dc.contributor.authorAktas, Yesim
dc.contributor.authorEsendagli, Gunes
dc.date.accessioned2024-08-04T20:50:14Z
dc.date.available2024-08-04T20:50:14Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBreast cancer is the most frequent cancer among women and impacts over two million women each year. Although many different types of anticancer agents are available for breast cancer treatment, doxorubicin is one of the most widely used drug. However, doxorubicin related side effects such as heart failure and arrhythmia limit its usage. To overcome this limitation and improve doxorubicin effectiveness, pegylated liposomal doxorubicin formulation Doxil (R)/Caelyx (R) was developed. Although cardiotoxicity related side effects were reduced with liposomal doxorubicin formulations, a superior effect was not obtained and better approaches are still needed. In this study, it was aimed to develop a more effective doxorubicin formulation than Doxil (R) and to evaluate its anticancer activity. In order to achieve this goal, small sized mesoporous silica nanoparticles (MSNs) (similar to 50 nm) were obtained, actively targeted with folic acid conjugation and loaded with doxorubicin. The obtained nanoparticles were fully characterized, conjugation was verified, and pH dependent drug release profile was shown. The nanoparticles' anticancer activity was investigated in detail on the ZR-75-1 and T47-D breast cancer cell lines. Fluorescence microscope and flow cytometry studies revealed that the cellular uptake of doxorubicin could be enhanced with small sized MSNs. Moreover, folic acid conjugation made a tangible contribution to this effect. Additionally, similar results were also obtained in cytotoxicity studies on both cell lines. In conclusion, actively targeted small sized MSNs may be a promising approach to potentiate the anticancer effect of doxorubicin.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK), Turkey [216S999]; Research Fund of the Inonu University, Turkey [TCD20212229]en_US
dc.description.sponsorshipGraphical abstract was produced using ChemDraw 15 Software (PerkinElmer, USA) . FTIR analyses were performed in ILKO ARGEM. This work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) , Turkey Project Number: 216S999 and Research Fund of the Inonu University, Turkey Project Number: TCD20212229. We thank Erciyes University Proofreading & Editing Office for English language editing.en_US
dc.identifier.doi10.1016/j.jddst.2021.102535
dc.identifier.issn1773-2247
dc.identifier.issn2588-8943
dc.identifier.scopus2-s2.0-85106278076en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jddst.2021.102535
dc.identifier.urihttps://hdl.handle.net/11616/99941
dc.identifier.volume63en_US
dc.identifier.wosWOS:000670348100001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Drug Delivery Science and Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDrug deliveryen_US
dc.subjectMesoporous silica nanoparticlesen_US
dc.subjectFolic aciden_US
dc.subjectActive targetingen_US
dc.subjectDoxorubicinen_US
dc.subjectDoxil (R)/Caelyx (R)en_US
dc.titleFolic acid decoration of mesoporous silica nanoparticles to increase cellular uptake and cytotoxic activity of doxorubicin in human breast cancer cellsen_US
dc.typeArticleen_US

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