Preparation and Characterization of Amino-Functionalized Zeolite/SiO2 Materials for Trypsin-Chymotrypsin Co-immobilization

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dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authoridAteş, Burhan/0000-0001-6080-229X
dc.authoridSEZER, Selda/0000-0002-3136-1250
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.authorwosidAteş, Burhan/AAA-3730-2021
dc.authorwosidSEZER, Selda/GQZ-6467-2022
dc.contributor.authorDogan, Demet
dc.contributor.authorSezer, Selda
dc.contributor.authorUlu, Ahmet
dc.contributor.authorKoytepe, Suleyman
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:50:13Z
dc.date.available2024-08-04T20:50:13Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractInorganic supports have attracted increased attention in enzyme immobilization since they not only improve enzyme stability but also reduce the final cost of enzymatic reactions. Herein, we explored the suitability of the amino-functionalized zeolite/SiO2 materials to co-immobilize trypsin-chymotrypsin mixture. For this purpose, the trypsin-chymotrypsin mixture was co-immobilized on the amino-functionalized zeolite/SiO2 materials and the immobilization yield was 80.7 +/- 7.6%. The pre-support and its modification were characterized by several techniques. Besides, the charges of the materials were investigated by zeta potentials at pH 5.0. As expected, the zeta potentials shifted from - 24.4 to - 8.16 mV after amino functionalization. Following immobilization, whereas the optimum pH (9.0) was not changed, the optimum temperature shifted from 50 to 40 degrees C. On the other hand, the immobilized trypsin-chymotrypsin showed comparatively higher thermal stability and storage stability than the soluble trypsin-chymotrypsin. The kinetic parameters were also calculated, however, while no significant change was observed in V-max, K-m value increased, which means that the affinity of enzyme to the substrate decreased after immobilization. Most strikingly, the residual activity of immobilized trypsin-chymotrypsin was 58% after eight repeated cycles. In conclusion, the preliminary experiments inferred that the amino-functionalized zeolite/SiO2 particles can be suitable and helpful support for trypsin-chymotrypsin immobilization. Graphicen_US
dc.identifier.doi10.1007/s10562-021-03636-2
dc.identifier.endpage2477en_US
dc.identifier.issn1011-372X
dc.identifier.issn1572-879X
dc.identifier.issue8en_US
dc.identifier.scopus2-s2.0-85104829631en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2463en_US
dc.identifier.urihttps://doi.org/10.1007/s10562-021-03636-2
dc.identifier.urihttps://hdl.handle.net/11616/99900
dc.identifier.volume151en_US
dc.identifier.wosWOS:000643587700001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofCatalysis Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectZeoliteen_US
dc.subjectSiO2 nanoparticlesen_US
dc.subjectAmino functionalizationen_US
dc.subjectTrypsin-chymotrypsinen_US
dc.subjectEnzyme immobilizationen_US
dc.titlePreparation and Characterization of Amino-Functionalized Zeolite/SiO2 Materials for Trypsin-Chymotrypsin Co-immobilizationen_US
dc.typeArticleen_US

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