Genotyping of Mycobacterium tuberculosis clinical isolates in two cities of Turkey

Basit Öğe Kaydı
dc.authoridAllix-Béguec, Caroline/0000-0001-7642-4844
dc.authoridSola, Christophe N/0000-0003-4672-2140
dc.authoridRASTOGI, NALIN/0000-0002-7199-7747
dc.authoridALP, ALPASLAN/0000-0001-7856-7590
dc.authoridGünal, Selami/0000-0002-4752-5176
dc.authoridDURMAZ, RIZA/0000-0001-6561-778X
dc.authorwosidAllix-Béguec, Caroline/AAI-4645-2021
dc.authorwosidDURMAZ, Rıza/HJH-4918-2023
dc.authorwosidSola, Christophe N/A-7992-2016
dc.authorwosidRASTOGI, NALIN/AAP-1958-2020
dc.authorwosidALP, ALPASLAN/I-8366-2013
dc.authorwosidGünal, Selami/AAA-7246-2021
dc.contributor.authorZozio, T
dc.contributor.authorAllix, C
dc.contributor.authorGunal, S
dc.contributor.authorSaribas, Z
dc.contributor.authorAlp, A
dc.contributor.authorDurmaz, R
dc.contributor.authorFauville-Dufaux, M
dc.date.accessioned2024-08-04T20:14:57Z
dc.date.available2024-08-04T20:14:57Z
dc.date.issued2005
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground: Population-based bacterial genetics using repeated DNA loci is an efficient approach to study the biodiversity and phylogeographical structure of human pathogens, such as Mycobacterium tuberculosis, the agent of tuberculosis. Indeed large genetic diversity databases are available for this pathogen and are regularly updated. No population-based polymorphism data were yet available for M. tuberculosis in Turkey, at the crossroads of Eurasia. Results: A total of 245 DNAs from Mycobacterium tuberculosis clinical isolates from tuberculosis patients residing in Turkey (Malatya n = 147 or Ankara n = 98) were genotyped by spoligotyping, a high-throughput genotyping method based on the polymorphism of the Direct Repeat locus. Thirty-three spoligotyping-defined clusters including 206 patients and 39 unique patterns were found. The ST41 cluster, as designated according to the international SpolDB3 database project, represented one fourth and when gathered to three genotypes, ST53, ST50 and ST284, one half of all the isolates. Out of 34 clinical isolates harboring ST41 which were further genotyped by IS6110 and by MIRU-VNTR typing, a typical 2-copy IS6110-RFLP pattern and a 215125113322 MIRU-VNTR pattern were observed among 21 clinical isolates. Further search in various databases confirms the likely Turkish-phylogeographical specificity of this clonal complex. Conclusion: We described a new phylogeographically-specific clone of M. tuberculosis, designated LAM7-TUR. Further investigations to assess its frequency within all regions of Turkey and its phylogeographical origin and phylogenetic tree will shed new light on its endemicity in Asia Minor.en_US
dc.identifier.doi10.1186/1471-2180-5-44
dc.identifier.issn1471-2180
dc.identifier.pmid16045794en_US
dc.identifier.scopus2-s2.0-24044497297en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1186/1471-2180-5-44
dc.identifier.urihttps://hdl.handle.net/11616/94074
dc.identifier.volume5en_US
dc.identifier.wosWOS:000231469900001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBmcen_US
dc.relation.ispartofBmc Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectStrain Differentiationen_US
dc.subjectMolecular Epidemiologyen_US
dc.subjectGlobal Distributionen_US
dc.subjectDrug-Resistanceen_US
dc.subjectPopulationen_US
dc.subjectDiversityen_US
dc.subjectAnatoliaen_US
dc.subjectSequenceen_US
dc.subjectOriginen_US
dc.titleGenotyping of Mycobacterium tuberculosis clinical isolates in two cities of Turkeyen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu