Freeze-dried ABTS•+ method: A ready-to-use radical powder to assess antioxidant capacity of vegetable oils

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dc.authoridDurmaz, Gökhan/0000-0003-3423-8108
dc.authorwosidDurmaz, Gökhan/E-7121-2016
dc.contributor.authorDurmaz, Gokhan
dc.date.accessioned2024-08-04T20:35:51Z
dc.date.available2024-08-04T20:35:51Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractA new approach to 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS(center dot+)) assay was conducted. ABTS(center dot+) radical solution in water was obtained by using classical method of incubating ABTS with potassium peroxodisulphate. The resulting radical solution was freeze-dried and tested for its radical efficiency by comparing freshly prepared ABTS(center dot+) (FP-ABTS) via using standard antioxidants. A high correlation was observed between the results obtained from FP-ABTS and freeze-dried ABTS (FD-ABTS) assays. When it was stored in the freezer. FD-ABTS powder was found to keep its initial activity to a great extend over one month. For the measurement of antiradical capacity of oil samples. FD-ABTS was dissolved in methanol and diluted to desired concentrations with a chloroform-methanol mixture. Linearity and accuracy of the method was confirmed by measuring the antiradical capacity of stripped sunflower oil spiked with different concentrations of standard antioxidants. Antiradical capacity of 22 oil samples including some commonly consumed vegetable oils was also tested by the FD-ABTS method. (C) 2012 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.foodchem.2012.02.064
dc.identifier.endpage1663en_US
dc.identifier.issn0308-8146
dc.identifier.issn1873-7072
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84859797542en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1658en_US
dc.identifier.urihttps://doi.org/10.1016/j.foodchem.2012.02.064
dc.identifier.urihttps://hdl.handle.net/11616/95623
dc.identifier.volume133en_US
dc.identifier.wosWOS:000303847400075en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofFood Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject2,2 '-Azinobis-(3-ethylbenzothiazoline-6-sulphonate)en_US
dc.subjectFreeze-dryingen_US
dc.subjectVegetable oilsen_US
dc.subjectAntiradical capacityen_US
dc.titleFreeze-dried ABTS•+ method: A ready-to-use radical powder to assess antioxidant capacity of vegetable oilsen_US
dc.typeArticleen_US

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