Prevalence of Candida africana and Candida dubliniensis, in vulvovaginal candidiasis: First Turkish Candida africana isolates from vulvovaginal candidiasis

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dc.authoridGümral, Ramazan/0000-0002-2303-8234
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authorwosidGümral, Ramazan/IAR-4281-2023
dc.authorwosidGUMRAL, RAMAZAN/ABC-8277-2021
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.contributor.authorHazirolan, G.
dc.contributor.authorAltun, H. U.
dc.contributor.authorGumral, R.
dc.contributor.authorGursoy, N. C.
dc.contributor.authorOtlu, B.
dc.contributor.authorSancak, B.
dc.date.accessioned2024-08-04T20:43:12Z
dc.date.available2024-08-04T20:43:12Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIntroduction. Candida africana and C. dubliniensis are closely related species of C. albicans. Current phenotypic methods are not suitable to accurately distinguish all the species belonging to the C. albicans complex. Several molecular-based methods have recently been designed for discriminating among closely related Candida species. The aim of this study was to establish the prevalence of C. dubliniensis and C. africana in vulvovaginal samples with phenotypic and genotypic methods. Materials and Methods. We re-examined 376 vulvovaginal C. albicans complex isolates. All the isolates were identified with morphological features and HWP1 gene polymorphisms. ITS and D1 /D2 sequencing, carbohydrate assimilation, MALDI-TOF MS profiles and antifungal susceptibilities were evaluated for C. africana and C. dubliniensis isolates. Results. Of the 376 isolates, three C. africana and three C. dubliniensis isolates (0.8% and 0.8% prevalence, respectively) were identified by molecular methods (HPW1, ITS and Dl /D2) Phenotypically, C. africana differed from C. albicans and C. dubliniensis by formation of no/rare pseudohyphae, absence of chlamydospores and, the development of turquoise green colonies on CHROMagar. MALDI-TOF MS and API ID 32 C could not revealed C. africana isolates. C. africana and C. dubliniensis isolates showed very low MIC values for all the tested antifungals. Discussion. This first report of C. africana from Turkey provides additional data for epidemiological, phenotypic features and antimicrobial susceptibility profiles. This study also highlights the importance of using genotypic methods in combination with phenotypic methods. (C) 2017 Elsevier Masson SAS. All rights reserved.en_US
dc.identifier.doi10.1016/j.mycmed.2017.04.106
dc.identifier.endpage381en_US
dc.identifier.issn1156-5233
dc.identifier.issn1773-0449
dc.identifier.issue3en_US
dc.identifier.pmid28641919en_US
dc.identifier.scopus2-s2.0-85020922891en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage376en_US
dc.identifier.urihttps://doi.org/10.1016/j.mycmed.2017.04.106
dc.identifier.urihttps://hdl.handle.net/11616/97860
dc.identifier.volume27en_US
dc.identifier.wosWOS:000413795500012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMasson Editeuren_US
dc.relation.ispartofJournal De Mycologie Medicaleen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectC. dubliniensisen_US
dc.subjectC. africanaen_US
dc.subjectHPW 1 gene polymorphismsen_US
dc.subjectITS sequencingen_US
dc.subjectD1/D2 sequencingen_US
dc.titlePrevalence of Candida africana and Candida dubliniensis, in vulvovaginal candidiasis: First Turkish Candida africana isolates from vulvovaginal candidiasisen_US
dc.typeArticleen_US

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