Exploring minocycline's effect on retinal degeneration following N-methyl-N-nitrosourea exposure in rats

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dc.contributor.authorKarabulut, Burak
dc.contributor.authorEroksuz, Hatice
dc.contributor.authorEroksuz, Yesari
dc.contributor.authorGul, Mehmet
dc.date.accessioned2026-04-04T13:32:58Z
dc.date.available2026-04-04T13:32:58Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractRetinal degeneration (RD) is often associated with deficiencies or the inaccurate production of photoreceptor-specific proteins, which are encoded by various genes and characterised by the apoptotic and ongoing death of photoreceptor cells. This study involved administering a single intraperitoneal (i.p.) dose of 50 mg/kg of N-methyl-N-nitrosourea (MNU) to rats to induce RD. Some of these rats also received intraperitoneal minocyc line at varying doses to prevent RD. Euthanasia was conducted at five intervals: at 12, 24, 48, and 72 h, and on the 7th day; and eye samples were taken. These samples were analysed using histopathology, immunohistochemistry, and electron microscopy. Significant RD was observed in the MNU-treated groups, with photoreceptor cell apoptosis demonstrated by the TUNEL method. Compared with those in the control group, there was a progressive thinning of the photoreceptor layer and outer nuclear layer, along with increased levels of glial fibrillary acidic protein (GFAP) and proliferating cell nuclear antigen (PCNA), and reduced levels of rhodopsin and red/green opsin starting from the 12th hour in the experimental groups. Electron microscopy revealed that amacrine and bipolar cells, in addition to photoreceptors, were also affected. The minocycline treatment did not show significant differences in retinal layer thickness or the staining levels of PCNA, GFAP, and opsins in the MNU-induced RD model.
dc.description.sponsorshipScientific and Technical Research Council of Turkiye (TUBITAK) , Scientific Research Projects, Fund-3001 [116O850]
dc.description.sponsorshipSupported by the Scientific and Technical Research Council of Turkiye (TUBITAK) , Scientific Research Projects, Fund-3001 (Project No. 116O850) .
dc.identifier.doi10.17221/122/2024-VETMED
dc.identifier.endpage260
dc.identifier.issn0375-8427
dc.identifier.issn1805-9392
dc.identifier.issue7
dc.identifier.orcid0000-0002-4907-6159
dc.identifier.pmid40809949
dc.identifier.scopus2-s2.0-105016126022
dc.identifier.scopusqualityQ3
dc.identifier.startpage247
dc.identifier.urihttps://doi.org/10.17221/122/2024-VETMED
dc.identifier.urihttps://hdl.handle.net/11616/108838
dc.identifier.volume70
dc.identifier.wosWOS:001539978100003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherCzech Academy Agricultural Sciences
dc.relation.ispartofVeterinarni Medicina
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectminocycline
dc.subjectretinal degeneration
dc.subjectretinitis pigmentosa
dc.titleExploring minocycline's effect on retinal degeneration following N-methyl-N-nitrosourea exposure in rats
dc.typeArticle

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