Urinary Multiomics Signatures of Preterm Premature Rupture of Membranes: An Exploratory Analysis of Microbial and Metabolic Biomarkers

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dc.contributor.authorDogan, Berat
dc.contributor.authorMumcu, Akin
dc.contributor.authorArda Duz, Senem
dc.contributor.authorOtlu, Baris
dc.contributor.authorKaraer, Abdullah
dc.contributor.authorTuncay, Gorkem
dc.date.accessioned2026-04-04T13:34:44Z
dc.date.available2026-04-04T13:34:44Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractPreterm premature rupture of membranes (PPROM) is a significant obstetric complication, often associated with infection-related processes. However, the underlying mechanisms remain poorly understood, particularly regarding the interplay between microbial dysbiosis and host metabolism. To address this, urine samples from women with PPROM and healthy controls were analyzed using 16S rRNA gene sequencing and 1H NMR-based metabolomics. Microbiota analysis revealed increased abundance of Hoylesella, Escherichia, Pseudomonas, and Enterococcus in the PPROM group, whereas Lactobacillus and Limosilactobacillus dominated in controls. Metabolomics identified key metabolites with diagnostic potential. Receiver operating characteristic (ROC) analysis showed that hippurate, formate, citrate, glycolate, serine, valine, and isoleucine had high discriminatory accuracy (AUC > 0.7), while beta-glucose, asparagine, pyroglutarate, 2-hydroxyglutarate, tyrosine, creatinine, and imidazole had moderate predictive power. The PPROM group exhibited increased valine, isoleucine, asparagine, and beta-glucose, while others decreased compared to controls. Multiomics integration revealed robust correlations between specific bacterial species and urinary metabolites, suggesting interactions between microbial dysbiosis and host metabolic pathways. These findings demonstrate distinct microbial and metabolic signatures in the urine of women with PPROM and support the utility of urinary multiomics analysis in advancing understanding of PPROM pathophysiology.
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK) [120C152]
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkiye (TUBITAK) with Project Number: 120C152.
dc.identifier.doi10.1021/acs.jproteome.5c00727
dc.identifier.endpage6237
dc.identifier.issn1535-3893
dc.identifier.issn1535-3907
dc.identifier.issue12
dc.identifier.orcid0000-0003-4810-1970
dc.identifier.pmid41252732
dc.identifier.scopus2-s2.0-105024014690
dc.identifier.scopusqualityQ1
dc.identifier.startpage6226
dc.identifier.urihttps://doi.org/10.1021/acs.jproteome.5c00727
dc.identifier.urihttps://hdl.handle.net/11616/109372
dc.identifier.volume24
dc.identifier.wosWOS:001618344900001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofJournal of Proteome Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250329
dc.subjectpreterm premature rupture of membranes
dc.subjectPPROM
dc.subject16S rRNA
dc.subjectH-1 NMR
dc.subjectmetabolomics
dc.subjectmultiomics
dc.subjecturine
dc.titleUrinary Multiomics Signatures of Preterm Premature Rupture of Membranes: An Exploratory Analysis of Microbial and Metabolic Biomarkers
dc.typeArticle

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