Histopathological, Ultrastructural and Apoptotic Changes in Diabetic Rat Placenta

dc.contributor.authorGül, Mehmet
dc.contributor.authorBayat, Nuray
dc.contributor.authorÇetin, Aslı
dc.contributor.authorKepekci, Remziye Aysun
dc.contributor.authorŞimşek, Yavuz
dc.contributor.authorKayhan, Başak
dc.contributor.authorTurhan, Uğur
dc.contributor.authorOtlu, Ali
dc.date.accessioned2020-06-09T11:01:02Z
dc.date.available2020-06-09T11:01:02Z
dc.date.issued2015-06
dc.departmentİnönü Üniversitesien_US
dc.descriptionBALKAN MEDICAL JOURNAL Volume: 32 Issue: 3 Pages: 296-302en_US
dc.description.abstractBackground: The exchange of substances between mother and fetus via the placenta plays a vital role during development. A number of developmental disorders in the fetus and placenta are observed during diabetic pregnancies. Diabetes, together with placental apoptosis, can lead to developmental and functional disorders. Aims: Histological, ultrastructural and apoptotic changes were investigated in the placenta of streptozotocin (STZ) induced diabetic rats. Study Design: Animal experimentation. Methods: In this study, a total of 12 female Wistar Albino rats (control (n=6) and diabetic (n=6)) were used. Rats in the diabetic group, following the administration of a single dose of STZ, showed blood glucose levels higher than 200 mg/dL after 72 hours. When pregnancy was detected after the rats were bred, two pieces of placenta and the fetuses were collected on the 20th day of pregnancy by cesarean incision under ketamine/xylazine anesthesia from in four rats from the control and diabetic groups. Placenta tissues were processed for light microscopy and transmission electron microscopy (TEM). Hematoxylin-eosin (HE) and periodic acid Schiff-diastase (PAS-D) staining for light microscopic and caspase-3 staining for immunohistochemical investigations were performed for each placenta. Electron microscopy was performed on thin sections contrasted with uranyl acetate and lead nitrate. Results: Weight gain in the placenta and fetuses of diabetic rats and thinning of the decidual layer, thickening of the hemal membrane, apoptotic bodies, congestion in intervillous spaces, increased PAS-D staining in decidual cells and caspase-3 immunoreactivity were observed in the diabetic group. After the ultrastructural examination, the apoptotic appearance of the nuclei of trophoblastic cells, edema and intracytoplasmic vacuolization, glycogen accumulation, dilation of the endoplasmic reticulum and myelin figures were observed. In addition, capillary basement membrane thickening, capillary endothelial cells chromatin condensation in the nucleus and corrugation of the nucleus were found. Conclusion: Diabetes causes histomorphometric, ultrastructural and apoptotic changes in rat placentaen_US
dc.description.sponsorshipInonu University -- BAP 2011/168en_US
dc.identifier.citationGul, M., Bayat, N., Cetin, A., Kepekci, R.A., Simsek, Y., Kayhan, B., Turhan, U., Otlu, A. Histopathological, Ultrastructural and Apoptotic Changes in Diabetic Rat Placenta. (2015).BALKAN MEDICAL JOURNAL.en_US
dc.identifier.doi10.5152/balkanmedj.2015.15290en_US
dc.identifier.endpage302en_US
dc.identifier.issn2146-3123
dc.identifier.issn2146-3131
dc.identifier.issue3en_US
dc.identifier.pmid26185719en_US
dc.identifier.startpage296en_US
dc.identifier.trdizinid173633en_US
dc.identifier.urihttps://doi.org/10.5152/balkanmedj.2015.15290
dc.identifier.urihttps://hdl.handle.net/11616/15671
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/173633
dc.identifier.volume32en_US
dc.identifier.wosWOS:000357330600010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherGALENOS YAYINCILIKen_US
dc.relation.ispartofBalkan Medical Journalen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosisen_US
dc.subjectultrastructureen_US
dc.subjecthistopathologyen_US
dc.subjectdiabetesen_US
dc.subjectplacentaen_US
dc.titleHistopathological, Ultrastructural and Apoptotic Changes in Diabetic Rat Placentaen_US
dc.typeArticleen_US

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