Ratlarda sisplatin ile oluşturulan deneysel akut böbrek hasarında H2S donörü NaHS ve kükürtlü kayısının koruyucu etkinliğinin araştırılması
Küçük Resim Yok
Tarih
2023
Yazarlar
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Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada, ratlarda sisplatin ile oluşturulan deneysel akut böbrek hasarında Hidrojen Sülfür (H2S) donörü Sodyum Hidrosülfür (NaHS) ve kükürtlü kuru kayısının koruyucu etkinliğinin araştırılması amaçlanmıştır. Materyal ve Metod: 40 adet (250-350g, 3-5 aylık, erkek) Wistar Albino sıçanlardan her grupta 10 sıçan olacak şekilde dört grup halinde gruplandırıldı: Kontrol grubu, sisplatin (20 mg/kg) grubu, siplatin+kükürtlü kayısı grubu ve sisplatin+NaHS (100 ?mol/kg) grubu olarak belirlendi. SOD, CAT, GPx ve CSE enzimlerin aktiviteleri ile MDA, GSH ve GSSG düzeyleri spektrofotometrik yöntemler kullanılarak manuel olarak ölçüldü. Kreatinin, Üre, Na, Cl, K, Ca düzeyleri ile AST ve ALT düzeyleri biyokimya otoanalizörü yardımıyla kit kullanılarak ölçüldü. Kan antiplatelet aktivite ölçümü Chrono Log Antiplatelet Analyzer 200 sistemi kullanılarak ristosetin ve adenozin difosfat (ADP) kiti yardımıyla ölçüldü. Böbrek dokularının histolojik incelemeleri yapıldı. Bulgular: Kontrol grubuna göre sisplatin grubunda böbrek dokusunda antioksidan enzim aktivitelerinde (CAT, CSE) ile MDA düzeyinde istatiksel olarak anlamlı sonuçlar elde edilmiştir. Aynı durum SOD ve GPx enzim aktivitelerinde de gözlenmiş, ancak istatiksel olarak anlamlı bulunamamıştır. Kan serumu ALT, AST, kreatinin ve üre düzeylerinde kontrol grubuna göre sisplatin grubunda istatiksel olarak anlamlı bir artış gözlenmiştir (p<0.05). ADP ve ristosetin aktivite sonuçlarında; ADP aktivitesinde kontrol grubuna göre sisplatin grubunda anlamlı bir şekilde azalırken (p<0.05), ristosetinde ise anlamlı bir sonuç gözlenememiştir. Histolojik bulgular çalışmamızda elde ettiğimiz biyokimyasal sonuçlarımızı desteklemektedir. Sonuç: Tüm bu sonuçlar değerlendirildiğinde sisplatinin böbrek üzerinde oluşturduğu hasarın önlenmesi açısından antioksidan etkisi bilinen kükürtlü kayısının ve H2S donörü olan NaHS kullanımının faydalı etkiler oluşturacağı kanısına varılmıştır. Anahtar Kelimeler: Akut böbrek hasarı, Hidrojen sülfür, Kükürtlü kayısı, Sisplatin
Aim: In this study, it was aimed to investigate the protective efficacy of H2S donor NaHS and sulphurous dried apricots in experimental acute kidney injury induced by cisplatin in rats. Material and Method: 40 Wistar Albino rats (250-350g, 3-5 months old, male) were grouped into four groups with 10 rats in each group: Control group, cisplatin (20 mg/kg) group, ciplatin+sulphurous apricot group and cisplatin+NaHS (100 ?mol/kg) group. The activities of SOD, CAT, GPx and CSE enzymes and the levels of MDA, GSH and GSSG were measured manually using spectrophotometric methods. Creatinine, Urea, Na, Cl, K, Ca levels and AST and ALT levels were measured using the kit with the help of a biochemistry autoanalyzer. Blood antiplatelet activity measurement was measured using the Chrono Log Antiplatelet Analyzer 200 system with the help of ristocetin and adenosine diphosphate (ADP) kit. Histological examinations of kidney tissues were performed. Results: Statistically significant results were obtained in antioxidant enzyme activities (CAT, CSE) and MDA level in kidney tissue in the cisplatin group compared to the control group. The same situation was observed in SOD and GPx enzyme activities, but it was not found to be statistically significant. A statistically significant increase was observed in the blood serum ALT, AST, creatinine and urea levels in the cisplatin group compared to the control group (p<0.05). ADP and ristocetin activity results; While ADP activity decreased significantly in the cisplatin group compared to the control group (p<0.05), no significant result was observed in ristocetin. Histological findings support our biochemical results obtained in our study. Conclusion: When all these results were evaluated, it was concluded that the use of sulphurous apricots with known antioxidant effects and NaHS, which is an H2S donor, will have beneficial effects in terms of preventing the damage caused by cisplatin on the kidney. Keywords: Acute kidney injury, Hydrogen sulfide, Sulphurous apricot, Cisplatin
Aim: In this study, it was aimed to investigate the protective efficacy of H2S donor NaHS and sulphurous dried apricots in experimental acute kidney injury induced by cisplatin in rats. Material and Method: 40 Wistar Albino rats (250-350g, 3-5 months old, male) were grouped into four groups with 10 rats in each group: Control group, cisplatin (20 mg/kg) group, ciplatin+sulphurous apricot group and cisplatin+NaHS (100 ?mol/kg) group. The activities of SOD, CAT, GPx and CSE enzymes and the levels of MDA, GSH and GSSG were measured manually using spectrophotometric methods. Creatinine, Urea, Na, Cl, K, Ca levels and AST and ALT levels were measured using the kit with the help of a biochemistry autoanalyzer. Blood antiplatelet activity measurement was measured using the Chrono Log Antiplatelet Analyzer 200 system with the help of ristocetin and adenosine diphosphate (ADP) kit. Histological examinations of kidney tissues were performed. Results: Statistically significant results were obtained in antioxidant enzyme activities (CAT, CSE) and MDA level in kidney tissue in the cisplatin group compared to the control group. The same situation was observed in SOD and GPx enzyme activities, but it was not found to be statistically significant. A statistically significant increase was observed in the blood serum ALT, AST, creatinine and urea levels in the cisplatin group compared to the control group (p<0.05). ADP and ristocetin activity results; While ADP activity decreased significantly in the cisplatin group compared to the control group (p<0.05), no significant result was observed in ristocetin. Histological findings support our biochemical results obtained in our study. Conclusion: When all these results were evaluated, it was concluded that the use of sulphurous apricots with known antioxidant effects and NaHS, which is an H2S donor, will have beneficial effects in terms of preventing the damage caused by cisplatin on the kidney. Keywords: Acute kidney injury, Hydrogen sulfide, Sulphurous apricot, Cisplatin
Açıklama
Anahtar Kelimeler
Biyokimya, Biochemistry
