Regulatory neuropeptides (ghrelin, obestatin and nesfatin-1) levels in serum and reproductive tissues of female and male rats with fructose-induced metabolic syndrome

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dc.authoridCatak, Zekiye/0000-0003-1973-3645
dc.authoridkuloğlu, tuncay/0000-0001-9874-3838
dc.authoridDagli, Adile Ferda/0000-0003-4077-4134;
dc.authorwosidŞahin, İbrahim/HHY-8303-2022
dc.authorwosidCatak, Zekiye/AAC-7181-2019
dc.authorwosidkuloğlu, tuncay/W-2310-2018
dc.authorwosidDagli, Adile Ferda/W-7878-2018
dc.authorwosidAKSOY, Aziz AKSOY/AAK-1775-2020
dc.contributor.authorCatak, Zekiye
dc.contributor.authorAydin, Suleyman
dc.contributor.authorSahin, Ibrahim
dc.contributor.authorKuloglu, Tuncay
dc.contributor.authorAksoy, Aziz
dc.contributor.authorDagli, Adile Ferda
dc.date.accessioned2024-08-04T20:39:40Z
dc.date.available2024-08-04T20:39:40Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAlthough, the exact mechanisms underlying the development of the metabolic syndrome (MetS) are not still completely understood, obesity, circulated peptide hormone levels and their interaction with genetic factors are considered largely responsible. The purpose of this study is to explore how the levels of ghrelin, obestatin (OBS) and NUCB2/nesfatin-1 (NES)/NUCB2 change in serum and the reproductive tissues of female and male rats with fructose-induced metabolic syndrome, and whether the levels of each hormone is correlated with the hormones involved with fertility. Experiments were conducted on 5-week-old Sprague-Dawley male and female rats assigned to either a control group or a MetS group. Controls were fed standard rat food and water ad libitum, while the MetS group was fed standard food with 10% (v/v) fructose solution added to their drinking water for 12 weeks with a 12/12 h photoperiod circle. Then, all animals were sacrificed after a one night fast. Peptides levels in the serum and reproductive tissues of rats were studied using the ELISA method while the immunoreactivity of reproductive system peptide hormones were shown by immunohistochemical staining method. Furthermore, the other biochemical parameters were measured using Konelab-60 equipment and infertility hormones were measured with Immulite2000. Fasting serum insulin, glucose, triglyceride, alanine aminotransferase (ALT), gamma glutamyl transpeptidase (GGT), low-density lipoprotein cholesterol (LDL-C), and total cholesterol (TC) levels were statistically significantly higher, and the amount of high density lipoprotein cholesterol (HDL-C) was significantly lower, in the MetS groups. Serum and tissue supernatant NES levels were significantly higher in the rats with MetS than the control group. Ghrelin, OBS and NES were expressed in the cytoplasm, concentrated around the apical parts of the epithelial cells in the reproductive tissues of the rats. The amounts of ghrelin were lower in the reproductive tissues of the animals with MetS, while NES levels in the same tissues increased. Obestatin also decreased, though not in the seminal glands. (C) 2014 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipFUBAP [1927]en_US
dc.description.sponsorshipThis study was sponsored by project number 1927 of FUBAP. The authors express their gratitude to Firat University.en_US
dc.identifier.doi10.1016/j.npep.2014.04.002
dc.identifier.endpage177en_US
dc.identifier.issn0143-4179
dc.identifier.issn1532-2785
dc.identifier.issue3en_US
dc.identifier.pmid24786976en_US
dc.identifier.scopus2-s2.0-84899915926en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage167en_US
dc.identifier.urihttps://doi.org/10.1016/j.npep.2014.04.002
dc.identifier.urihttps://hdl.handle.net/11616/96433
dc.identifier.volume48en_US
dc.identifier.wosWOS:000336825000007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherChurchill Livingstoneen_US
dc.relation.ispartofNeuropeptidesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMetabolic syndromeen_US
dc.subjectGhrelinen_US
dc.subjectObestatinen_US
dc.subjectNUCB2/nesfatin-1en_US
dc.subjectInfertilityen_US
dc.titleRegulatory neuropeptides (ghrelin, obestatin and nesfatin-1) levels in serum and reproductive tissues of female and male rats with fructose-induced metabolic syndromeen_US
dc.typeArticleen_US

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