Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR-RFLP method with the potential use for other Echinococcus species

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dc.authoridDagli, Adile Ferda/0000-0003-4077-4134
dc.authoridÖZERCAN, ibrahim Hanifi/0000-0002-8781-8838
dc.authoridCetinkaya, Ulfet/0000-0001-5527-3741
dc.authorwosidKuk, Salih/ABA-7923-2020
dc.authorwosidDagli, Adile Ferda/W-7878-2018
dc.authorwosidÖZERCAN, ibrahim Hanifi/W-7883-2018
dc.authorwosidCetinkaya, Ulfet/AAL-6965-2021
dc.contributor.authorSakalar, Cagri
dc.contributor.authorKuk, Salih
dc.contributor.authorErensoy, Ahmet
dc.contributor.authorDagli, Adile Ferda
dc.contributor.authorOzercan, Ibrahim Hanifi
dc.contributor.authorCetinkaya, Ulfet
dc.contributor.authorYazar, Suleyman
dc.date.accessioned2024-08-04T20:39:49Z
dc.date.available2024-08-04T20:39:49Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground/aim: To develop a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol using a new genomic marker sequence and a novel set of restriction enzymes in order to detect and discriminate 2 Echinococcus species, E. granulosus and E. multilocularis, found in formalin-fixed paraffin-embedded (FFPE) human tissues. Materials and methods: DNA was isolated from 11 FFPE human tissue samples positive for cystic echinococcosis or alveolar echinococcosis. A mitochondrial genomic marker region was amplified and sequenced using a novel primer pair and a new PCR-RFLP protocol was developed for the detection and discrimination of E. granulosus and E. multilocularis using a set of restriction enzymes including AccI, MboI, MboII, and TsoI. Results: The selected marker region was amplified using DNA isolated from FFPE human tissue samples positive for cystic echinococcosis or alveolar echinococcosis and the discrimination of E. granulosus and E. multilocularis was accomplished by use of the novel PCR-RFLP method. Conclusion: In this PCR-RFLP protocol, use of any single restriction enzyme is enough for the discrimination of E. granulosus and E. multilocularis. The PCR-RFLP protocol can be potentially used for the discrimination of 5 other Echinococcus species: E. oligarthus, E. shiquicus, E. ortleppi, E. canadensis, and E. vogeli.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [110S001]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK project number 110S001). We thank Dr Bruno Gottstein for providing the genomic DNA of E. multilocularis.en_US
dc.identifier.doi10.3906/sag-1303-114
dc.identifier.endpage748en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue5en_US
dc.identifier.pmid25539539en_US
dc.identifier.scopus2-s2.0-84905656585en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage741en_US
dc.identifier.urihttps://doi.org/10.3906/sag-1303-114
dc.identifier.urihttps://hdl.handle.net/11616/96530
dc.identifier.volume44en_US
dc.identifier.wosWOS:000343068800005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFFPE tissuesen_US
dc.subjectEchinococcus granulosusen_US
dc.subjectEchinococcus multilocularisen_US
dc.subjectsequencingen_US
dc.subjectPCR-RFLPen_US
dc.titleMolecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR-RFLP method with the potential use for other Echinococcus speciesen_US
dc.typeArticleen_US

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