Immobilization of Trametes trogii laccase on polyvinylpyrrolidone-coated magnetic nanoparticles for biocatalytic degradation of textile dyes

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dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authoridAtes, Burhan/0000-0001-6080-229X
dc.authoridBoran, Filiz/0000-0002-8801-7987
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.contributor.authorBakar, Busra
dc.contributor.authorBirhanli, Emre
dc.contributor.authorUlu, Ahmet
dc.contributor.authorBoran, Filiz
dc.contributor.authorYesilada, Ozfer
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:53:25Z
dc.date.available2024-08-04T20:53:25Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractHigh cost and low operational stability are the most important challenges limiting the possible use of laccase in the removal of textile dyes. To overcome these challenges, in this study, polyvinylpyrrolidone (PVP)-coated magnetic nanoparticles (MNPs) were produced and characterized. To our knowledge, this is the first study to explore the feasibility of immobilizing a Trametes trogii laccase enzyme on Fe3O4/PVP MNPs. The characterization of samples and the successful immobilization of laccase were verified by characterization methods. Besides, the biochemical properties and stability of the Fe3O4/PVP/Lac were evaluated in terms of optimum pH, optimum temperature, thermostability, thermodynamic and kinetic parameters, storage stability, operational stability, and decolorization efficiency of two different textile dyes. The optimum activities were recorded at pH 2.5 degrees C and 30 degrees C. The Fe3O4/PVP/Lac displayed remarkable thermal stability and activation energy for denaturation, enthalpy, Gibbs free energy, and entropy results confirmed the enhanced stability of Fe3O4/PVP/Lac against high temperatures. Meanwhile, the Fe3O4/PVP/Lac retained about 58% of its original activity after seven consecutive reuses, while it retained up to 25% of its original activity after 28 d of storage at room temperature. K-m and V-max for the Fe3O4/PVP/Lac were calculated to be 126 mu M and 211 mu mol/min, respectively. Finally, after 8 and 6 cycles of repeated use, the Fe3O4/PVP/Lac still decolorized 32.34% and 32.23% of Remazol Brilliant Blue R (RBBR) and Indigo Carmine (IC), respectively. As envisioned, this study suggests a promising way to solve the problems of high price and poor operational stability of the enzyme during biocatalytic decolorization of textile dyes in wastewaters.en_US
dc.identifier.doi10.1080/10242422.2023.2173006
dc.identifier.endpage211en_US
dc.identifier.issn1024-2422
dc.identifier.issn1029-2446
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85147767389en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage194en_US
dc.identifier.urihttps://doi.org/10.1080/10242422.2023.2173006
dc.identifier.urihttps://hdl.handle.net/11616/101166
dc.identifier.volume42en_US
dc.identifier.wosWOS:000928722100001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBiocatalysis and Biotransformationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMagnetic nanoparticlesen_US
dc.subjectpolyvinylpyrrolidoneen_US
dc.subjectlaccase immobilizationen_US
dc.subjectRemazol Brilliant Blue Ren_US
dc.subjectIndigo Carmineen_US
dc.subjectdye decolorizationen_US
dc.titleImmobilization of Trametes trogii laccase on polyvinylpyrrolidone-coated magnetic nanoparticles for biocatalytic degradation of textile dyesen_US
dc.typeArticleen_US

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