Evaluation of human interferon adsorption performance of Cibacron Blue F3GA attached cryogels and interferon purification by using FPLC system

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dc.authoridUzun, Lokman/0000-0002-3971-7725
dc.authorwosid/ABI-3951-2020
dc.authorwosidDenizli, Adil/G-5151-2012
dc.contributor.authorDogan, Ali
dc.contributor.authorOzkara, Serpil
dc.contributor.authorSari, Mufrettin Murat
dc.contributor.authorUzun, Lokman
dc.contributor.authorDenizli, Adil
dc.date.accessioned2024-08-04T20:35:51Z
dc.date.available2024-08-04T20:35:51Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn this study, we have focused our attention on preparing supermacroporous cryogels as a potential dye-affinity adsorbent for interferon purification. For this purpose, 2-hydroxyethyl methacrylate (HEMA) and Cibacron Blue F3GA (CB) were selected as main monomer and dye-ligand. Cibacron Blue F3GA attached supermacroporous poly(2-hydroxyethyl methacrylate) [poly( HEMA)/CB] cryogels were prepared and characterized by swelling test, scanning electron microscopy, elemental analysis, and FTIR. After that, the effecting factors such as pH, concentration, interaction time, and ionic strength on the interferon separation were evaluated. The maximum adsorption capacity of poly(HEMA)/CB cryogels was obtained as 38.2 mg/g at pH 6.0. Fast protein liquid chromatography (FPLC) system was used for interferon purification from human gingival fibroblast extract. The chromatography parameters, capacity and selectivity factors, resolution and theoretical plate number were found as 7.79, 9.62, 4.23 and 554, respectively. Although some decreases in total protein content, from 320 mu g to 18 mu g, and interferon activity, from 2.6 x 10(3) IU to 2.2 x 10(3) IU, were determined, specific antiviral activity increased from 7.19 IU/mu g to 122.2 IU/mu g. The purified interferon samples have 97.6% purity determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After repeated ten adsorption-desorption cycles, no significant decrease was determined in adsorption capacity of cryogel. In result, poly(HEMA)/CB cryogels have an application potential for rapid, cheap and specific purification of interferon. (C) 2012 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.jchromb.2012.02.036
dc.identifier.endpage76en_US
dc.identifier.issn1570-0232
dc.identifier.issn1873-376X
dc.identifier.pmid22445306en_US
dc.identifier.scopus2-s2.0-84859442417en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage69en_US
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2012.02.036
dc.identifier.urihttps://hdl.handle.net/11616/95614
dc.identifier.volume893en_US
dc.identifier.wosWOS:000303088600010en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Chromatography B-Analytical Technologies in The Biomedical and Life Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPoly(HEMA) cryogelen_US
dc.subjectCibacron Blue F3GAen_US
dc.subjectInterferonen_US
dc.subjectAffinity purificationen_US
dc.subjectFast protein liquid chromatographyen_US
dc.titleEvaluation of human interferon adsorption performance of Cibacron Blue F3GA attached cryogels and interferon purification by using FPLC systemen_US
dc.typeArticleen_US

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