Enhanced production of laccase in repeated-batch cultures of Funalia trogii and Trametes versicolor

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dc.authoridYesilada, Ozfer/0000-0003-0038-6575
dc.authorwosidYesilada, Ozfer/ABI-1335-2020
dc.contributor.authorBirhanli, Emre
dc.contributor.authorYesilada, Ozfer
dc.date.accessioned2024-08-04T20:32:31Z
dc.date.available2024-08-04T20:32:31Z
dc.date.issued2010
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe biotechnologically important enzyme laccase (benzenediol: oxygen oxidoreductase: EC 1.10.3.2) is secreted by white rot fungi. However, these organisms produce insufficient amount of laccase for use in various biotechnological areas. The main aim of this study is to enhance the laccase production in the repeated-batch cultures of Funalia trogii ATCC 200800 and Trametes versicolor ATCC 200801 isolated in Turkey. In this study, laccase production in the repeated-batch cultures of F. trogii and T. versicolor pre-grown pellets were investigated under various conditions. Retention time, temperature, agitation, pH and the amount of pellets were found to be important for laccase production during repeated-batch studies. The culture filtrate showed one protein band. Zymogram gel also showed only one band of activity. The enzyme remained fully active when frozen for 300 clays. The immobilized fungi were also able to produce high amounts of laccase during reuse. It is possible to obtain high laccase amounts with free and immobilized repeated-batch cultures under the most appropriate culture conditions determined. Considering the various biotechnological applications of laccase, an enhancement in laccase production through the selection of appropriate culture conditions could facilitate the development of more economical and environmentally friendly processes. (C) 2010 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipInonu University Research Funden_US
dc.description.sponsorshipWe thank Inonu University Research Fund Unit for financial support of this study. We also thank Dr. Mustafa Sahiner for English corrections.en_US
dc.identifier.doi10.1016/j.bej.2010.06.019
dc.identifier.endpage37en_US
dc.identifier.issn1369-703X
dc.identifier.issn1873-295X
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-77956266800en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage33en_US
dc.identifier.urihttps://doi.org/10.1016/j.bej.2010.06.019
dc.identifier.urihttps://hdl.handle.net/11616/95117
dc.identifier.volume52en_US
dc.identifier.wosWOS:000282407200005en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofBiochemical Engineering Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectImmobilizationen_US
dc.subjectLaccaseen_US
dc.subjectPelleten_US
dc.subjectRepeated-batchen_US
dc.subjectWhite rot fungien_US
dc.titleEnhanced production of laccase in repeated-batch cultures of Funalia trogii and Trametes versicoloren_US
dc.typeArticleen_US

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