HEV seroprevalence in blood donors in Turkey by two commercial total anti-HEV Ab ELISA kits

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dc.authoridKarahan, Zeynep Ceren/0000-0001-7727-3363
dc.authoridDELİALİOĞLU, Rabia ALBAYRAK/0000-0002-1969-4319
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridKızılpınar, Mehtap/0000-0001-5943-9147
dc.authoridYurdcu, Esra/0000-0002-1441-6408
dc.authorwosidKarahan, Zeynep Ceren/ACC-1836-2022
dc.authorwosidDELİALİOĞLU, Rabia ALBAYRAK/AAF-9484-2020
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.authorwosidKızılpınar, Mehtap/AAQ-6458-2020
dc.authorwosidYurdcu, Esra/E-5802-2019
dc.contributor.authorYasar, Osman
dc.contributor.authorKaratayli, Ersin
dc.contributor.authorCengiz, Guniz
dc.contributor.authorKizilpinar, Mehtap
dc.contributor.authorYurdcu, Esra
dc.contributor.authorAlbayrak, Rabia
dc.contributor.authorGuven, Aysel
dc.date.accessioned2024-08-04T20:46:44Z
dc.date.available2024-08-04T20:46:44Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPrevious hepatitis E virus (HEV) seroprevalence studies in Turkey have shown high variabilities, leading to conflicting results. We aimed to re-evaluate HEV seroprevalence among blood donors in Turkey using the Wantai (Beijing, China) and the Dia.Pro (Milan, Italy) total anti-HEV antibody (Ab) enzyme-linked immunosorbent assay (ELISA) kits and compare their performances and to investigate the presence of HEV RNA in blood donors. Serum total anti-HEV antibodies were determined in a total of 2011 volunteer blood donor samples collected from different regions of Turkey (807 from Ankara, 243 from Kayseri, 284 from Izmir, 200 from Malatya, 200 from Kahramanmaras, and 277 from Van). HEV RNA was evaluated by a real-time polymerase chain reaction in a total of 272 anti-HEV seropositive samples. The country-wide HEV seroprevalence was calculated as 11.5% (Dia.Pro) and 12.2% (Wantai) with seropositivity rates of 12.0%-12.5% in Ankara, 7.4%-8.2% in Kayseri, 14.5%-15.5% in Malatya, 8.1%-8.8% in Izmir, 15.0%-16.0% in Kahramanmaras, and 12.6%-13.4% in Van by Dia.Pro and Wantai kits, respectively. The lowest detectable Ab concentrations were 0.16 and 0.14 units/mL WHO, for the Dia.Pro and the Wantai assays, respectively, showing no significant difference between assays. HEV RNA was not detected in any of the anti-HEV seropositive samples. Compared with previous studies, HEV was shown to have a higher overall seroprevalence in Turkey. Despite its limitation, the current study represents the most comprehensive HEV seroprevalence study in Turkey performed with two different commercial ELISA assays with high sensitivities so far. Further investigation is required to determine HEV genotypes in Turkey.en_US
dc.description.sponsorshipAnkara University, Scientific Research Projects [14B0207001]en_US
dc.description.sponsorshipAnkara University, Scientific Research Projects, Grant/Award Number: 14B0207001en_US
dc.identifier.doi10.1002/jmv.25567
dc.identifier.endpage2181en_US
dc.identifier.issn0146-6615
dc.identifier.issn1096-9071
dc.identifier.issue12en_US
dc.identifier.pmid31403185en_US
dc.identifier.scopus2-s2.0-85070924175en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage2174en_US
dc.identifier.urihttps://doi.org/10.1002/jmv.25567
dc.identifier.urihttps://hdl.handle.net/11616/98917
dc.identifier.volume91en_US
dc.identifier.wosWOS:000482484500001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Medical Virologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectELISAen_US
dc.subjecthepatitis E virusen_US
dc.subjectseroprevalenceen_US
dc.subjecttotal anti-HEV Aben_US
dc.subjectTurkeyen_US
dc.subjectviral hepatitisen_US
dc.titleHEV seroprevalence in blood donors in Turkey by two commercial total anti-HEV Ab ELISA kitsen_US
dc.typeArticleen_US

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