Ziconotide inhibits KCL-induced increase in intracellular calcium in rat sensory neurons: A peripheral mechanism of analgesic action

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dc.authorscopusid55936522900
dc.authorscopusid7102067803
dc.authorscopusid23134421500
dc.authorscopusid18038758400
dc.authorscopusid36671738100
dc.authorscopusid54681943600
dc.authorscopusid7005883729
dc.contributor.authorKuzgun T.K.
dc.contributor.authorÖzcan M.
dc.contributor.authorAlçin E.
dc.contributor.authorSerhatlio?lu I.
dc.contributor.authorÖzcan S.
dc.contributor.authorKaçar E.
dc.contributor.authorAyar A.
dc.date.accessioned2024-08-04T20:00:54Z
dc.date.available2024-08-04T20:00:54Z
dc.date.issued2013
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjective: Ziconotide, a novel non-opioid intrathecal analgesic agent with higher analgesic activity than morphine, has been recently approved for the treatment of severe and cronic pain. However, the exact cellular mechanism of the analgesic effects of ziconotide is not well documented. The aim of this study was to investigate the effects of ziconotide on KCl-induced intracellular calcium ([Ca2+]i) signaling in cultured rat dorsal root ganglion (DRG) neurons. Material and Methods: DRG neurons were grown in primary culture, following enzymatic and mechanical dissociation of ganglia from 1 or 2-day-old Wistar rats. DRG neurons were loaded with calcium-sensitive dye Fura-2 AM (1 ?mol). Initialy calcium-sensitive dye loaded cells were stimulated with application of KCl (30 mM) alone (control). Then, ziconotide (1 nM,10 nM and 1 ?M)+KCl were applied. Finally, these cells were stimulated with application of KCl again and intracellular calcium ([Ca2+]i) responses were quantified by the changes in the ratio of 340/380 nm using fluorescence calcium imaging system. Results: Ziconotide [1 nM (n=17), 10 nM (n=23) and 1 ?M (n=37)] dose-dependently reduced the increase of [Ca2+]i which was elicited by 30 mM KCl (high K+) in a rather irreversible manner. Moreover, pretreatment with 1 ?M ziconotide effectively prevented [Ca2+]i response to the high K+. Conclusion: It was concluded that ziconotide reduced the membrane depolarisation-induced increase in [Ca2+]i, in concentration dependent and a partially irreversible manner in rat DRG neurons, providing evidence for peripheral antinociceptive action of this drug. The effects on [Ca2+]i may indicate that ziconotide reduces release of pro-nociceptive neurochemicals in the peripheral pain pathway. © 2013 by Türkiye Klinikleri.en_US
dc.identifier.doi10.5336/medsci.2012-32552
dc.identifier.endpage1265en_US
dc.identifier.issn1300-0292
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-84888156722en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage1259en_US
dc.identifier.urihttps://doi.org/10.5336/medsci.2012-32552
dc.identifier.urihttps://hdl.handle.net/11616/91099
dc.identifier.volume33en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTurkiye Kliniklerien_US
dc.relation.ispartofTurkiye Klinikleri Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCalciumen_US
dc.subjectGangliaen_US
dc.subjectPainen_US
dc.subjectSpinalen_US
dc.subjectZiconotideen_US
dc.titleZiconotide inhibits KCL-induced increase in intracellular calcium in rat sensory neurons: A peripheral mechanism of analgesic actionen_US
dc.title.alternativeZikonotid si{dotless}çan duyusal nöronlari{dotless}nda KCL ile İndüklenen hücre İçi kalsiyum arti{dotless}şi{dotless}ni{dotless} İnhibe eder: Analjezik etkinli?in bir periferal mekanizmasi{dotless}en_US
dc.typeArticleen_US

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