Sıçan prefrontal korteksinde tolüenin neden olduğu apoptoza karşı melatoninin koruyucu etkisinin araştırılması
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Tarih
2012
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info:eu-repo/semantics/openAccess
Özet
Öz: Tolüen endüstride sık kullanılan, periferik ve santral sinir sistemi hasarına yol açabilen solunabilir bir hidrokarbondur. Pineal bezin en temel hormonu olan melatonin güçlü bir antioksidan ve radikal giderici özelliği sahiptir. Bu çalışma tolüen solunumunun sıçan prefrontal korteksi üzerindeki apoptotik etkisini ve melatonin hormonunun koruyucu etkisini araştırmak amacı ile planlandı. Gereç ve Yöntem: Bu amaçla 21 adet Wistar-Albino cinsi erkek sıçan (200-220 g) 3 eşit gruba ayrıldı. Grup I kontrol olarak kullanıldı. Grup II’ deki sıçanlar dört hafta boyunca tolüene maruz bırakılırken (3000 ppm/1 saat/gün) Grup III’deki sıçanlara tolüen ile birlikte melatonin verildi (10 mg/kg/gün, ip). Dört haftalık deney süresinin sonunda hayvanlar dekapitasyon yolu ile öldürüldü. Prefrontal korteks dokusu çıkarılarak %10’luk formalin solüsyonuna atıldı. Doku örnekleri parafine gömüldü ve kesildi (5 ?m kalınlıkta). Apoptozisin tespit edilebilmesi için kesitler TUNEL yöntemi ile boyandı. Bulgular: Tolüen soluyan sıçanların prefrontal korteks dokusunda apoptotik hücre sayısının arttığı görüldü. Melatonin tedavisinin apoptotik hücre sayısını belirgin bir şekilde azalttığı görüldü.
Abstract: Objective: Toluene is a volatile hydrocarbon that is principally used in industry and can cause central and peripheral nervous system impairment. Melatonin, the principal secretory product of the pineal gland, functions as a potent antioxidant and free radical scavenger. This study was designed to investigate the harmful effects of toluene inhalation and protective effects of melatonin in the prefrontal cortex of rats. Material and Methods: For this purpose, 21 adult male Wistar-albino rats (200-220 g) were randomly divided into three equal groups. Animals in group I were used as control group. The rats in group II were exposed toluene (3000 ppm/1hour/day) for 4 weeks, while the rats in group III treated with melatonin (10 mg/kg/day, ip) plus toluene inhalation. At the end of 4 weeks experimental period, all rats were killed by decapitation. The prefrontal cortex tissues were removed and fixed in % 10 neutral formalin solution. Then tissue specimens were embedded in paraffin and sectioned (thickness, 5μm). The sections were immunohistochemically stained using TUNEL method for determination apoptosis. Results: Increased numbers of apoptotic cell were observed in the prefrontal cortex of toluene inhaled rats. Treatment of melatonin markedly reduced the the number of apoptotic cells. Conclusion: In conclusion, in the present immunohistochemical study, we found that melatonin treatment prevents toluene-induced neuronal damage in the prefrontal cortex of rats.
Abstract: Objective: Toluene is a volatile hydrocarbon that is principally used in industry and can cause central and peripheral nervous system impairment. Melatonin, the principal secretory product of the pineal gland, functions as a potent antioxidant and free radical scavenger. This study was designed to investigate the harmful effects of toluene inhalation and protective effects of melatonin in the prefrontal cortex of rats. Material and Methods: For this purpose, 21 adult male Wistar-albino rats (200-220 g) were randomly divided into three equal groups. Animals in group I were used as control group. The rats in group II were exposed toluene (3000 ppm/1hour/day) for 4 weeks, while the rats in group III treated with melatonin (10 mg/kg/day, ip) plus toluene inhalation. At the end of 4 weeks experimental period, all rats were killed by decapitation. The prefrontal cortex tissues were removed and fixed in % 10 neutral formalin solution. Then tissue specimens were embedded in paraffin and sectioned (thickness, 5μm). The sections were immunohistochemically stained using TUNEL method for determination apoptosis. Results: Increased numbers of apoptotic cell were observed in the prefrontal cortex of toluene inhaled rats. Treatment of melatonin markedly reduced the the number of apoptotic cells. Conclusion: In conclusion, in the present immunohistochemical study, we found that melatonin treatment prevents toluene-induced neuronal damage in the prefrontal cortex of rats.
Açıklama
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Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi
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Scopus Q Değeri
Cilt
26
Sayı
1
Künye
TAŞ U,ÖGETÜRK M,IRMAK H. S,KARACA Z. İ,ÖZYURT B,SÖĞÜT E,SARSILMAZ M (2012). Sıçan prefrontal korteksinde tolüenin neden olduğu apoptoza karşı melatoninin koruyucu etkisinin araştırılması. Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi, 26(1), 1 - 6.
