Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR- RFLP method with the potential use for other Echinococcus species

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dc.contributor.authorSakalar, Çağrı
dc.contributor.authorKuk, Salih
dc.contributor.authorErensoy, Ahmet
dc.contributor.authorDağlı, Adile Ferda
dc.contributor.authorÖzercan, İbrahim Hanifi
dc.contributor.authorÇetinkaya, Ülfet
dc.contributor.authorYazar, Süleyman
dc.date.accessioned2024-08-04T19:54:38Z
dc.date.available2024-08-04T19:54:38Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground/aim: To develop a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol using a new genomic marker sequence and a novel set of restriction enzymes in order to detect and discriminate 2 Echinococcus species, E. granulosus and E. multilocularis, found in formalin-fixed paraffin-embedded (FFPE) human tissues. Materials and methods: DNA was isolated from 11 FFPE human tissue samples positive for cystic echinococcosis or alveolar echinococcosis. A mitochondrial genomic marker region was amplified and sequenced using a novel primer pair and a new PCR-RFLP protocol was developed for the detection and discrimination of E. granulosus and E. multilocularis using a set of restriction enzymes including AccI, MboI, MboII, and TsoI. Results:The selected marker region was amplified using DNA isolated fromFFPE human tissue samples positive for cystic echinococcosis or alveolar echinococcosis and the discrimination of E. granulosus and E. multilocularis was accomplished by use of the novel PCR-RFLP method. Conclusion: In this PCR-RFLP protocol, use of any single restriction enzyme is enough for the discrimination of E. granulosus and E. multilocularis. The PCR-RFLP protocol can be potentially used for the discrimination of 5 other Echinococcus species: E. oligarthus, E. shiquicus, E. ortleppi, E. canadensis, and E. vogeli.en_US
dc.identifier.endpage748en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue5en_US
dc.identifier.startpage741en_US
dc.identifier.trdizinid213163en_US
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/213163
dc.identifier.urihttps://hdl.handle.net/11616/89997
dc.identifier.volume44en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleMolecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR- RFLP method with the potential use for other Echinococcus speciesen_US
dc.typeArticleen_US

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