Neuroprotective effects of pregabalin in experimental spinal cord injury: An investigation of oxidative stress and antioxidant enzymes in blood and neural tissue

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dc.contributor.authorGudu, Burhan Oral
dc.contributor.authorEseoglu, Metehan
dc.contributor.authorTurkoz, Yusuf
dc.contributor.authorGul, Mehmet
dc.contributor.authorDogan, Zumrut
dc.date.accessioned2026-04-04T13:33:00Z
dc.date.available2026-04-04T13:33:00Z
dc.date.issued2026
dc.departmentİnönü Üniversitesi
dc.description.abstractBACKGROUND: This study aimed to evaluate the neuroprotective potential of pregabalin (PB) and methylprednisolone (MP) in a rat model of spinal cord injury (SCI) by assessing serum and spinal cord levels of superoxide dismutase (SOD) and glutathione peroxidase (GPx), markers of oxidative stress, and neurological recovery outcomes. METHODS: Forty-four rats were randomized into six groups: sham, PB control (40 mg/kg), SCI alone, MP-treated SCI (30 mg/kg), and PB-treated SCI (40 and 80 mg/kg). SCI was induced at the T10 level using the Allen weight-drop method. PB and MP were administered intraperitoneally for three days post-injury. Neurological recovery was assessed using the Tarlov scale and inclined plane test. Although 44 rats were initially allocated, mortality and technical loss resulted in a final cohort of 35 animals; however, post hoc power remained >90% for key biochemical outcomes. RESULTS: SOD levels were significantly reduced in the MP+SCI group compared with the sham (p=0.006), SCI (p=0.015), 40 PB (p=0.004), and 80 PB+SCI (p=0.028) groups. Additionally, the SCI group exhibited lower SOD activity than the 40 PB group (p=0.007). Serum glutathione peroxidase levels were significantly lower in both the SCI (p=0.018) and 80 PB+SCI (p=0.009) groups compared with the sham group, whereas the 40 PB group showed higher GPx activity than the SCI (p=0.010) and 80 PB+SCI (p=0.006) groups. In spinal cord tissue, SOD activity in the 40 PB+SCI group was significantly lower than in the SCI group (p=0.007). Additionally, SOD activity in the SCI group was significantly higher than in the 40 PB group (p=0.007). Spinal cord GPx levels were significantly elevated in the SCI group compared with the sham (p=0.007), MP+SCI (p=0.010), 40 PB (p=0.003), 40 PB+SCI (p=0.003), and 80 PB+SCI (p=0.028) groups. Furthermore, the MP+SCI group demonstrated higher GPx activity than the sham group (p=0.045). Pregabalin improved inclined-plane performance but did not produce significant changes in Tarlov motor scores, indicating selective enhancement of postural stability rather than full locomotor recovery. Histopathological analysis revealed no significant differences between the trauma groups. CONCLUSION: Pregabalin mitigated oxidative stress and partially improved functional stability in experimental spinal cord injury, suggesting possible clinical applicability pending further validation.
dc.identifier.doi10.14744/tjtes.2025.35346
dc.identifier.endpage17
dc.identifier.issn1306-696X
dc.identifier.issn1307-7945
dc.identifier.issue1
dc.identifier.orcid0000-0002-5011-815X
dc.identifier.pmid41821277
dc.identifier.scopus2-s2.0-105029008891
dc.identifier.scopusqualityQ2
dc.identifier.startpage9
dc.identifier.urihttps://doi.org/10.14744/tjtes.2025.35346
dc.identifier.urihttps://hdl.handle.net/11616/108868
dc.identifier.volume32
dc.identifier.wosWOS:001691464600002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Assoc Trauma Emergency Surgery
dc.relation.ispartofUlusal Travma Ve Acil Cerrahi Dergisi-Turkish Journal of Trauma & Emergency Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectPregabalin
dc.subjectspinal cord injury
dc.subjectsuperoxide dismutase
dc.subjectglutathione peroxidase
dc.subjectantioxidant
dc.subjectmethylprednisolone
dc.titleNeuroprotective effects of pregabalin in experimental spinal cord injury: An investigation of oxidative stress and antioxidant enzymes in blood and neural tissue
dc.typeArticle

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