Fruktozun oluşturduğu metabolik sendromda kardiyak akt/enos yolağı üzerine lactobacillus plantarum'un etkisinin incelenmesi
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Dosyalar
Tarih
2022
Yazarlar
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Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Diyette yaygın şekilde kullanılan fruktozun, metabolik sendrom gelişme riskini artırdığı gösterilmiştir. Metabolik sendrom oksidatif stres ve inflamatuvar değişkenlerle ilişkili olarak kardiyovasküler hastalık riskini artırır. Laktik asit bakterilerinden olan Lactobacillus plantarum (L. plantarum)'un, antioksidan, antikanser, antiinflamatuvar, antiproliferatif, obezite karşıtı ve antidiyabetik etkileri bulunmaktadır. Laktik asit bakterilerinin probiyotik suşlarının fruktoz ile metabolik sendrom oluşturulan modellerde kardiyovasküler hastalıklar üzerine etkisini inceleyen sınırlı sayıda çalışma yer almaktadır. Çalışmamızda, L. plantarum takviyesinin, fruktozla beslenen sıçanların kalplerinde, Akt/eNOS yolağı üzerindeki etkisi araştırılarak mekanizma irdelenmiştir. Materyal ve Metot: Bu çalışma, fruktoz ile metabolik sendrom geliştirilmiş Wistar Albino erkek sıçanlarda yürütüldü. Sıçanlar kontrol, fruktoz ve fruktoz+L. plantarum olmak üzere üç gruba ayrıldı. Fruktoz, 15 hafta süre ile içme suyu içerisinde %20'lik çözelti şeklinde verilirken; probiyotikler, fruktoz uygulamasının son 6 haftasında günde bir kez (109 CFU/ml/100 g) olmak üzere gastrik gavaj ile uygulandı. Kalp dokularındaki Akt, eNOS, iNOS, IRS-1, SIRT1, mTOR, NF-κB ve TNF-α protein düzeyleri Western Blot analiz yöntemleri ile değerlendirildi. Bulgular: L. plantarum uygulamasının fruktoz grubuna göre sıçanların kalp dokusunda Akt düzeyini %24.7 (p<0.0001), IRS-1 düzeyini %43.7 (p=0.0113), eNOS düzeyini %25.7 (p<0.0001), mTOR düzeyini %35.1 (p<0.0001), SIRT 1 düzeyini %41.7 (p=0.0031) oranında yükselttiği ve iNOS düzeyini %25.0 (p<0.0001), NF-κB düzeyini %27.6 (p<0.0001), TNF-α düzeyini ise %26.1 (p<0.0001) oranında düşürdüğü tespit edildi. Sonuç: Çalışma, L. plantarum'un kalpte meydana gelen yangıyı engelleyebileceği, Akt/eNOS yolağı aracılığıyla insülin duyarlılığının arttırılmasına katkı sağlayabileceği ve metabolik sendrom kaynaklı oluşabilecek kardiyovasküler bozuklukların önüne geçebileceğini göstermiştir.
Aim: It has been shown that fructose, which is widely used in the diet, increases the risk of developing metabolic syndrome. Metabolic syndrome, on the other hand, increases the risk of cardiovascular disease in relation to oxidative stress and inflammatory variables. Lactobacillus plantarum (L. plantarum), one of the lactic acid bacteria, has antioxidant, anticancer, anti-inflammatory, antiproliferative, anti-obesity and antidiabetic effects. There are a limited number of studies in the literature examining the effects of probiotic strains of lactic acid bacteria on cardiovascular diseases in models with fructose-induced metabolic syndrome. In this study, it was investigated whether L. plantarum supplementation had an effect on the Akt/eNOS pathway in the hearts of fructose fed rats. Material and Method: This study was conducted in Wistar Albino male rats with fructose-induced metabolic syndrome. Rats control, fructose and fructose+L. plantarum were divided into three groups. While fructose is given as a 20% solution in drinking water for 15 weeks; probiotics were administered by gastric gavage once a day (109CFU/ml/100 g) in the last 6 weeks of fructose administration. Akt, eNOS, iNOS, IRS-1, SIRT1, mTOR, NF-kB and TNF-α protein levels in heart tissues were evaluated by Western blot analysis methods. Results: According to the fructose group of L. plantarum application, Akt level was 24.7% (p<0.0001), IRS-1 level was 43.7% (p=0.0113), eNOS level was 25.7% (p<0.0001), mTOR level was 35.1% (p<0.0001) in heart tissue of rats compared to fructose group. ) increased SIRT 1 level by 41.7% (p=0.0031) and increased iNOS level by 25.0% (p<0.0001), NF-kB level by 27.6% (p<0.0001), and TNF-α level by 26.1% (p<0.0001) found to be reduced. Conclusion: As a result of the study, it has been shown that L. plantarum can prevent heart inflammation, contribute to increasing insulin sensitivity through Akt/eNOS pathway, and prevent cardiovascular disorders that may occur due to metabolic syndrome.
Aim: It has been shown that fructose, which is widely used in the diet, increases the risk of developing metabolic syndrome. Metabolic syndrome, on the other hand, increases the risk of cardiovascular disease in relation to oxidative stress and inflammatory variables. Lactobacillus plantarum (L. plantarum), one of the lactic acid bacteria, has antioxidant, anticancer, anti-inflammatory, antiproliferative, anti-obesity and antidiabetic effects. There are a limited number of studies in the literature examining the effects of probiotic strains of lactic acid bacteria on cardiovascular diseases in models with fructose-induced metabolic syndrome. In this study, it was investigated whether L. plantarum supplementation had an effect on the Akt/eNOS pathway in the hearts of fructose fed rats. Material and Method: This study was conducted in Wistar Albino male rats with fructose-induced metabolic syndrome. Rats control, fructose and fructose+L. plantarum were divided into three groups. While fructose is given as a 20% solution in drinking water for 15 weeks; probiotics were administered by gastric gavage once a day (109CFU/ml/100 g) in the last 6 weeks of fructose administration. Akt, eNOS, iNOS, IRS-1, SIRT1, mTOR, NF-kB and TNF-α protein levels in heart tissues were evaluated by Western blot analysis methods. Results: According to the fructose group of L. plantarum application, Akt level was 24.7% (p<0.0001), IRS-1 level was 43.7% (p=0.0113), eNOS level was 25.7% (p<0.0001), mTOR level was 35.1% (p<0.0001) in heart tissue of rats compared to fructose group. ) increased SIRT 1 level by 41.7% (p=0.0031) and increased iNOS level by 25.0% (p<0.0001), NF-kB level by 27.6% (p<0.0001), and TNF-α level by 26.1% (p<0.0001) found to be reduced. Conclusion: As a result of the study, it has been shown that L. plantarum can prevent heart inflammation, contribute to increasing insulin sensitivity through Akt/eNOS pathway, and prevent cardiovascular disorders that may occur due to metabolic syndrome.
Açıklama
Anahtar Kelimeler
Eczacılık ve Farmakoloji
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WoS Q Değeri
Scopus Q Değeri
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Durmaz Kurşun, Ö. E. (2022). Fruktozun oluşturduğu metabolik sendromda kardiyak akt/enos yolağı üzerine lactobacillus plantarum'un etkisinin incelenmesi. İnönü Üniversitesi, Malatya.
