Immobilization of glucose oxidase in GLYMO/MTEO sol-gel film for glucose biosensor application
Küçük Resim Yok
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An electrochemical biosensor was developed by using the sol-gel coating solution. The modified platinum electrode used in the study was constructed by immobilization of glucose oxidase under a layer of sol-gel film. The coating solution was prepared by using GLYMO, and MTEOS. Electrochemical measurements were carried out amperometrically by determining hydrogen peroxide produced by the enzymatic reaction between glucose and glucose oxidase. It was observed that the amperometric response of the enzyme electrode was linear for glucose concentrations in the range from 2 to 10 mM. The response time of the biosensor to reach a steady-state current value was approximately 80 s. The glucose selectivity of the biosensor was determined in the presence of some interfering substances, such as lactose, sucrose, urea, uric acid, oxalic acid, and ascorbic acid. It was observed that the interfering molecules did not cause any observable signal. The stability of the sensor was investigated, and it was found hat the initial sensor response decreased approximately 44% over a period of 30 days of storage in dry state at 4 °C.
Açıklama
Anahtar Kelimeler
Biosensor, Glucose, Glucose oxidase, GLYMO, MTEOS, Sol-gel
Kaynak
Acta Chimica Slovenica
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
59
Sayı
2
