Immobilization of Xylanase on ZnO nanoparticles obtained by green synthesis from Eupatorium cannabinum L. and its application in enrichment of fruit juices

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dc.authorscopusid58095637000
dc.authorscopusid57214094703
dc.authorscopusid35436084000
dc.authorscopusid56997144400
dc.authorscopusid55362533700
dc.authorscopusid6507504123
dc.contributor.authorSelçuk Pekdemir S.
dc.contributor.authorBakar B.
dc.contributor.authorTaş R.
dc.contributor.authorUlu A.
dc.contributor.authorPekdemir M.E.
dc.contributor.authorAteş B.
dc.date.accessioned2024-08-04T20:03:41Z
dc.date.available2024-08-04T20:03:41Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractJuice production is one of the main components of the nutritional food industry. Therefore, improving fruit juice quality and preventing the formation of negative by-products is important for human health. The role of environmentally friendly, safe and economically more affordable green nanocarriers in improving the quality of fruit juice is increasing day by day. For this purpose, zinc oxide nanoparticles (ZnONPs) were prepared from the aqueous extract of the Eupatorium cannabinum L. using the green synthesis method. After immobilization, structural and morphological characterizations of the prepared ZnONPs and xylanase (Xyl)@ZnONPs were carried out, which confirmed that both the synthesis of ZnONPs and the immobilization of Xyl enzyme on ZnONPs were successfully carried out. The biochemical parameters of Xyl@ZnONPs such as optimum pH and temperature, kinetic parameters, thermal stability, reusability, and storage stability were investigated and compared with free Xyl. After immobilization, the optimum pH of free Xyl shifted from 5.0 to an alkaline pH of 8.0, while no change in the optimum temperature (70 °C) was observed for both enzymes. While the activation energy of free Xyl was 5.56 kJ/mol, it was found to be 7.27 kJ/mol for Xyl@ZnONPs. Xyl@ZnONPs outperformed free Xyl due to its long-term storage stability and reusability. Moreover, Xyl@ZnONPs retained 50 % of its activity after 5 reuses and 87 % of its activity after 4 weeks of storage at room temperature. In addition, a decrease in Km value and an increase in Vmax value were observed after immobilization, which means the increased catalytic activity of the Xyl@ZnONPs. As a result of re-using Xyl@ZnONPs in orange juice for 6 cycles, clarification yield of 66.43 % was obtained under optimized conditions. Overall, this study prepared a suitable, environmentally friendly, and robust carrier support for Xyl immobilization and developed a biocatalyst that will provide high performance in the industrial field. © 2024 Elsevier B.V.en_US
dc.description.sponsorshipYükseköğretim Kurulu; FBG-2021-2731en_US
dc.description.sponsorshipThe research work was supported by the Scientific Research Projects Unit of \u0130n\u00F6n\u00FC University (Project Number: FBG-2021-2731) and F\u0131rat University. B\u00FC\u015Fra Bakar is a 100/2000 the Council of Higher Education PhD Scholar in the Biomaterial and Tissue Engineering subdivision.en_US
dc.identifier.doi10.1016/j.mcat.2024.114232
dc.identifier.issn2468-8231
dc.identifier.scopus2-s2.0-85193203915en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.mcat.2024.114232
dc.identifier.urihttps://hdl.handle.net/11616/92024
dc.identifier.volume562en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofMolecular Catalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectFruit juice clarificationen_US
dc.subjectGreen synthesisen_US
dc.subjectXylanaseen_US
dc.subjectZnO nanoparticlesen_US
dc.titleImmobilization of Xylanase on ZnO nanoparticles obtained by green synthesis from Eupatorium cannabinum L. and its application in enrichment of fruit juicesen_US
dc.typeArticleen_US

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