Effect of Copper Staining in Wilson Disease: A Liver Explant Study

Simple item page
dc.authoridKaradag Soylu, Nese/0000-0002-6303-5484
dc.authoridTolan, Huseyin kerem/0000-0002-0845-8820
dc.authoridAKPOLAT, Nusret/0000-0002-9138-2117
dc.authoridYilmaz, Sezai/0000-0002-8044-0297
dc.authoridTurkmen, Samdanci, Emine/0000-0002-0034-5186
dc.authorwosidKaradag Soylu, Nese/ABH-9544-2020
dc.authorwosidTolan, Huseyin kerem/HJP-5645-2023
dc.authorwosidAKPOLAT, Nusret/ABF-6986-2020
dc.authorwosidYilmaz, Sezai/ABI-2323-2020
dc.authorwosidTurkmen, Samdanci, Emine/ABH-4716-2020
dc.contributor.authorKaradag, Nese
dc.contributor.authorTolan, Kerem
dc.contributor.authorSamdanci, Emine
dc.contributor.authorSelimoglu, Ayse
dc.contributor.authorAkpolat, Nusret
dc.contributor.authorYilmaz, Sezai
dc.date.accessioned2024-08-04T20:44:02Z
dc.date.available2024-08-04T20:44:02Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjectives: Wilson disease is a rare genetic disease with clinical and histopathologic differential diagnostic challenges. In this study, we evaluated the histo pathologic findings of explanted livers in Wilson disease, with special emphasis on copper histochemistry. Materials and Methods: Our study group was recruited by reviewing archived histopathology reports and the liver transplant clinic patient records retrospectively for patients who had liver transplant for Wilson disease between January 2010 and June 2015, at Turgut Ozal Medical Center. Archival slides were reevaluated. When needed, relevant clinical and laboratory data were obtained from patient medical records. Results: During the selected period, there were 33 patients fitting the study criteria (22 male, 11 female, mean age of 22 +/- 11 y). All patients had mild to moderate septal inflammation. We found that 29 patients (88%) showed glycogenated hepatocyte nuclei and 27 patients (79%) showed nuclear pleomorphism. Other histopathologic findings were cholestasis (48%) and macrovesicular steatosis (39%). There was no special finding in hilar regions except for 2 patients who had recanalized portal vein thrombosis. In terms of copper histochemistry, 2 copper stains, Timm silver sulfide and rhodanine, were performed in all cases, with orcein staining only done for 25 of the cases. Positivity rates for these copper stains were 85%, 82%, and 36%. Periodic acid-Schiff-diastase-and periodic acid-Schiff-positive granules were detected in 7 of 33 patients (21%). Iron deposition was seen in 12 patients (focal and/or minimal in 11, more than focal in 1). There was no dysplasia or malignancy in any of the patients. Conclusions. On routine hematoxylin and eosinstained slides, detection of glycogenated hepatocyte nuclei and the finding of the nuclear pleomorphism should alert the pathologist for the possibility of Wilson disease, especially with cryptogenic liver disease. Timm stain is a more convenient histo chemical stain in revealing copper deposition in liver.en_US
dc.identifier.doi10.6002/ect.2015.0319
dc.identifier.endpage546en_US
dc.identifier.issn1304-0855
dc.identifier.issn2146-8427
dc.identifier.issue5en_US
dc.identifier.pmid27759555en_US
dc.identifier.scopus2-s2.0-85030759010en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage542en_US
dc.identifier.urihttps://doi.org/10.6002/ect.2015.0319
dc.identifier.urihttps://hdl.handle.net/11616/97988
dc.identifier.volume15en_US
dc.identifier.wosWOS:000416088700011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBaskent Univen_US
dc.relation.ispartofExperimental and Clinical Transplantationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCopper histochemistryen_US
dc.subjectTransplanten_US
dc.subjectWilson diseaseen_US
dc.titleEffect of Copper Staining in Wilson Disease: A Liver Explant Studyen_US
dc.typeArticleen_US

Files

Collections

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu

We collect and process your personal information for the following purposes: Authentication, Preferences, Acknowledgement and Statistics.
To learn more, please read our privacy policy.

Customize