The knockdown of stathmin with si-RNA inhibits invasion of mesothelioma

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dc.authorwosidUlas, mustafa/KIE-9518-2024
dc.contributor.authorAksoy, Asude
dc.contributor.authorVaroglu, Asuman
dc.contributor.authorOnalan, Ebru Etem
dc.contributor.authorTektemur, Ahmet
dc.contributor.authorArtas, Gokhan
dc.contributor.authorKoc, Mustafa
dc.contributor.authorCakmak, Muharrem
dc.date.accessioned2024-08-04T20:55:00Z
dc.date.available2024-08-04T20:55:00Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground To investigate the mechanism of action of stathmin1 (STMN1) in mesothelioma (MSM) and whether it has any role in its treatment. Methods STMN1 expression was examined using immunohistochemistry in biopsy tissues taken from MSM patients. The relationships between the levels of STMN1 expression in the pathology preparations of MSM patients, and the clinicopathological characteristics of these patients, and their survival times were investigated. Transfection of STMN1-specific siRNA into SPC212 cells was compared to negative control siRNAs. The mRNA levels of genes that may play a role in invasion, apoptosis, and autophagy were evaluated by RT-PCR. Results The expression of STMN1 was shown to be high in MSM tissues (p < 0.05). It was found that the only independent predictor factor affecting the survival time of MSM patients was the disease stage (p < 0.05). STMN1 was significantly reduced after siRNA intervention (81.5%). STMN1 with specific siRNA has been shown to suppress invasion by reducing the mRNA levels of cadherin-6 (CDH6), fibroblast growth factor-8 (FGF8), hypoxia-inducible factor 1 (HIF1A), matrix metallopeptidase 1-2 (gelatinase A) (MMP1-2), and TIMP metallopeptidase inhibitor 2 (TIMP2), which are important markers for invasion. Although the expression of apoptosis and autophagy-related genes, caspase-2 (Casp2) and LC-3, was reduced by silencing STMN1 with specific siRNA in western blot analysis, this effect was not observed in PCR results. Conclusions Immunohistochemical analysis of STMN1 may contribute to the differential diagnosis of MSM, and STMN1 may also be considered as a potential therapeutic target in the early invasive stage of MSM therapy.en_US
dc.description.sponsorshipScientific Research Project of Firat University [TF.15.47/2015]en_US
dc.description.sponsorshipThe authors declared that this study has received financial support from the Scientific Research Project of Firat University (no: TF.15.47/2015) . This study was approved by the Ethical Committee at the University of Firat, Medical Faculty (11.10.2016, 17/03) .en_US
dc.identifier.doi10.1016/j.tice.2024.102303
dc.identifier.issn0040-8166
dc.identifier.pmid38244401en_US
dc.identifier.scopus2-s2.0-85183196485en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.tice.2024.102303
dc.identifier.urihttps://hdl.handle.net/11616/101779
dc.identifier.volume87en_US
dc.identifier.wosWOS:001177884200001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherChurchill Livingstoneen_US
dc.relation.ispartofTissue & Cellen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAutophagyen_US
dc.subjectApoptosisen_US
dc.subjectMesotheliomaen_US
dc.subjectInvasionen_US
dc.subjectStathmin1en_US
dc.subjectSurvivalen_US
dc.titleThe knockdown of stathmin with si-RNA inhibits invasion of mesotheliomaen_US
dc.typeArticleen_US

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