Voltammetric Determination of 5-Hydroxyindoleacetic Acid at Poly (p-amino benzene sulfonic acid) Modified Sensor
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Univ Tehran, Fac Chemistry, Center Excellence Electrochemistry
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, the quantitative amount of 5-hydroxyindoleacetic acid (5-HIAA) was determined at poly (p-amino benzene sulfonic acid) (p-ABSA) modified glassy carbon electrode. The redox property of 5-HIAA was investigated by cyclic voltammetry. The cyclic voltammograms showed that the redox reaction of 5-HIAA was irreversible. Scan rate study showed that the redox reaction of 5-HIAA was controlled by both diffusion and adsorption on the poly (p-ABSA) modified sensor. Differential pulse voltammetry technique was used for the quantitative analysis of 5-HIAA in phosphate buffer solution at pH 7.00. The linear working range of the calibration graphs was determined as 1x10(-5)-9x10(-5) M (R-2, 0.9912), and the detection limit was determined as 5.3x10(-7) M. Recovery values in the analysis of urine samples were between 99.4% and 103.0%. The results showed that the modified sensor can be applied to the determination of 5-HIAA in the presence of ascorbic acid. The proposed sensor is promising for routine analysis due to its high selectivity, reproducibility, long-term stability, and high recovery values in biological samples.
Açıklama
Anahtar Kelimeler
5-Hydroxyindolacetic acid, Serotonin, p-Amino benzene sulfonic acid, Differential pulse voltammetry, Cyclic voltammetry
Kaynak
Analytical & Bioanalytical Electrochemistry
WoS Q Değeri
N/A
Scopus Q Değeri
Q4
Cilt
14
Sayı
4
