Genetic engineering of Theileria parva lactate dehydrogenase gene: a new anti-theilerial target

Basit Öğe Kaydı
dc.authoridGeckil, Hikmet/0000-0003-0070-0691
dc.authorwosidTaskin, ırmak Icen/AAA-6149-2021
dc.authorwosidGeckil, Hikmet/F-7647-2012
dc.contributor.authorIcen-Taskin, Irmak
dc.contributor.authorMunzuroglu, Omer
dc.contributor.authorGeckil, Hikmet
dc.date.accessioned2024-08-04T20:45:21Z
dc.date.available2024-08-04T20:45:21Z
dc.date.issued2018
dc.departmentİnönü Üniversitesien_US
dc.description.abstractTheileria parva is the causative agent of East Coast Fever (ECF), a tick borne disease, which results in major economic losses in cattle. Major problems in dealing with this illness are the high cost of drugs, development of resistance, and absence of effective vaccines. Thus, exploiting new targets for cost effective and higher therapeutic value drugs are imperative. Glycolysis is the main pathway for generation of ATP in T. parva, given its development inside erythrocytes. Thus, the enzymes of this pathway may prove potential targets for designing new-generation anti-theilerials. Lactate dehydrogenase of T. parva (TpLDH} has the highest activity of all glycolytic enzymes and thus we selected this enzyme as the potential therapeutic target. Our study is the first to report the isolation, removal of introns through directed mutagenesis, and cloning of TpLDH and showing that amino acid insertions or deletions most notably corresponded to a 5-amino acid sequence (Asn-91A, Glu-91B, Glu-91C, Trp-91D, Asn-91E) between Ser-91 ve Arg-92 of the enzyme. This region is also present in other apicomplexan such as Babesia bovis, a pathogen of cattle and Plasmodium falciparum, a human pathogen. Providing as the attachment site for the enzyme inhibitors and not being present in LDH of respective hosts, we propose this site as an attractive drug target. The work here is expected to lead new studies on detailed structural and kinetic aspects of apicomplexan LDHs and development of new inhibitors.en_US
dc.description.sponsorshipFirat University Scientific Research Projects Unit (FUBAP) [FF.11.16]en_US
dc.description.sponsorshipWe thank Venhar Celik for her contribution to this study. We also thank Dr. Roger Pelle (International Livestock Research Institute, Nairobi, Kenya) for providing us the genomic DNA of the Theileria parva. This study has been supported by Firat University Scientific Research Projects Unit (FUBAP) with FF.11.16 project number.en_US
dc.identifier.doi10.1590/1678-5150-PVB-5116
dc.identifier.endpage888en_US
dc.identifier.issn0100-736X
dc.identifier.issn1678-5150
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85051048074en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage883en_US
dc.identifier.urihttps://doi.org/10.1590/1678-5150-PVB-5116
dc.identifier.urihttps://hdl.handle.net/11616/98415
dc.identifier.volume38en_US
dc.identifier.wosWOS:000440153300014en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherRevista Pesquisa Veterinaria Brasileiraen_US
dc.relation.ispartofPesquisa Veterinaria Brasileiraen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTheileria parvaen_US
dc.subjectlactate dehydrogenaseen_US
dc.subjectanti-theilerialen_US
dc.subjectEast coast feveren_US
dc.subjectparasiteen_US
dc.titleGenetic engineering of Theileria parva lactate dehydrogenase gene: a new anti-theilerial targeten_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu