Investigation of survivin gene polymorphism in patients with gastric carcinoma
Yükleniyor...
Dosyalar
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Dicle Medical Journal / Dicle Tip Dergisi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Dünya genelinde mide kanserinin insidansı düş-
mesine rağmen hala önemli bir sağlık problemidir. Türkiye’de
ise yılda 30.000 yeni mide kanseri vakasıyla 2. en
sık görülen kanserdir. Mide kanseri genetik ve çevresel
faktörlerin etkileşimiyle ortaya çıkan çok faktörlü karma-
şık bir hastalıktır. Kanserli dokuda aşırı ifade edilen survivin,
apoptozis inhibe edici proteinlerdendir. Bu çalışmada
Survivin -31 G/C polimorfizmi ile mide kanseri arasındaki
ilişki araştırıldı.
Gereç ve yöntem: Çalışma Düzce Üniversitesi Araştırma
ve Uygulama Hastanesi Patoloji Laboratuvarına gelen
mide kanseri tanısı konmuş 46 hasta ve sağlıklı bireylerin
oluşturduğu 42 kişilik kontrol grubu ile gerçekleştirildi. Bu
bireylerin genotipi Düzce Üniversitesi, Tıp Fakültesi, Tıbbi
Genetik Anabilim Dalı Laboratuvarlarında PCR-RFLP
yöntemiyle tayin edildi.
Bulgular: Hasta grubunda, GG genotipi 16 (% 34,8),
GC genotipi 21 (% 45,7) ve CC genotipi ise 9 (% 19,6)
olguda saptandı. Kontrol grubunda ise, genotip dağılımı
sırasıyla 13 (% 31), 26 (% 61,9) ve 3 (% 7,1) bulundu.
Hasta ve kontrol grubu karşılaştırıldığında istatistiksel
olarak anlamlı bir fark saptanamadı. Fakat CC genotipine
sahip bireylerin mide kanserine yakalanma riskinin GG
(OR=1,52) daha fazla risk oluşturduğu bulundu.
Sonuç: Bu çalışma bildiğimiz kadarıyla Türk toplumunda
mide kanseri ile Survivin -31G/C polimorfizmini araştıran
ilk çalışmadır. Elde ettiğimiz sonuçlar hasta ve kontrol
gruplarımızın temsil ettiği toplum kesitinde mide kanseri
ile Survivin -31 G/C polimorfizmi arasında anlamlı bir ilişki
olmadığını göstermekle birlikte CC genotipinin mide kanserine
yatkınlık oluşturduğu düşünülebilir.
Objectives: Despite decreasing incidence of gastric cancer in worldwide, it is still a major health problem. Every year, 30.000 new gastric cancer cases emerging, and it is the second most common cancer in Turkey. Gastric cancer is a complex multifactorial disease, emerging by interaction between genetic and environmental factors. Survivin, apoptosis inhibitory protein is over-expressed in cancer tissue. In this study, association between Survivin -31G/C polymorphism and gastric carcinoma was investigated. Materials and Methods: 46 gastric carcinoma patients who had been admitted at Düzce University Research and Practice Hospital, Laboratory of Pathology and 42 healthy individuals have been included in the study. Samples have been subjected to genetic analysis by PCRRFLP method in Medical Genetics Department laboratory at Düzce University. Results: GG genotype was found in 16 (34.8%), GC genotype in 21 (45.7%), CC genotype in 9 (19.6%) in patient group. In control group, genotype distribution were found 13 (31%), 26 (61.9%) and 3 (7.1%) respectively. The statistically significant difference was not found when compared between patient and control groups. However, we observed the increased occurrence of gastric cancer associated with CC genotype (OR=1.52). Conclusions: In our knowledge, this study is the first to evaluate the relationship between gastric carcinoma and Survivin -31G/C polymorphism in Turkish population. Our results show that there is no any association between gastric carcinoma and Survivin -31G/C polymorphism in the community which is represented by our study and control groups. However, it was concluded that CC genotype may create the susceptibility to gastric cancer.
Objectives: Despite decreasing incidence of gastric cancer in worldwide, it is still a major health problem. Every year, 30.000 new gastric cancer cases emerging, and it is the second most common cancer in Turkey. Gastric cancer is a complex multifactorial disease, emerging by interaction between genetic and environmental factors. Survivin, apoptosis inhibitory protein is over-expressed in cancer tissue. In this study, association between Survivin -31G/C polymorphism and gastric carcinoma was investigated. Materials and Methods: 46 gastric carcinoma patients who had been admitted at Düzce University Research and Practice Hospital, Laboratory of Pathology and 42 healthy individuals have been included in the study. Samples have been subjected to genetic analysis by PCRRFLP method in Medical Genetics Department laboratory at Düzce University. Results: GG genotype was found in 16 (34.8%), GC genotype in 21 (45.7%), CC genotype in 9 (19.6%) in patient group. In control group, genotype distribution were found 13 (31%), 26 (61.9%) and 3 (7.1%) respectively. The statistically significant difference was not found when compared between patient and control groups. However, we observed the increased occurrence of gastric cancer associated with CC genotype (OR=1.52). Conclusions: In our knowledge, this study is the first to evaluate the relationship between gastric carcinoma and Survivin -31G/C polymorphism in Turkish population. Our results show that there is no any association between gastric carcinoma and Survivin -31G/C polymorphism in the community which is represented by our study and control groups. However, it was concluded that CC genotype may create the susceptibility to gastric cancer.
Açıklama
Anahtar Kelimeler
Polimorfizm, Mide kanseri, Survivin gen, Apoptozis, Polymorphism, Gastric carcinoma, Surviving gene, Apoptosis
Kaynak
Dicle Medical Journal / Dicle Tip Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
39
Sayı
4
Künye
Yılmaz, N. Yaykaşlı, K. O. Soğuktaş, H. Oktay, M. Erdem, H. Kaya, E. Ekinci, A. Kaya, S.(2012). Investigation of survivin gene polymorphism in patients with gastric carcinoma. Dicle Medical Journal / Dicle Tip Dergisi, 39(4), 499–503.
