Integrated chemical and biological characterization of Hypericum perforatum extract using LC-MS/MS and in vitro functional assays

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dc.contributor.authorGuzel, Mehmet Ali
dc.contributor.authorKolac, Turgay
dc.contributor.authorMenevse, Irem Nur
dc.contributor.authorDundar, Muhammed
dc.contributor.authorZengin, Rukiye
dc.contributor.authorGuzel, Abdussamat
dc.contributor.authorUgur, Yilmaz
dc.date.accessioned2026-04-04T13:34:40Z
dc.date.available2026-04-04T13:34:40Z
dc.date.issued2026
dc.departmentİnönü Üniversitesi
dc.description.abstractHypericum perforatum L. (St. John's wort) is a medicinal plant known for its diverse secondary metabolites and pharmacological potential. This study provides a comprehensive evaluation of the phytochemical composition and bioactivities of methanolic extracts from wild H. perforatum. LC-MS/MS analysis identified 36 compounds, including phenolic acids (chlorogenic acid, protocatechuic acid) and flavonoids (rutin, isoquercitrin, quercetin), with the first-time detection of genkwanin, vicenin-2, schaftoside, and afzelin, to the best of our knowledge. The extract demonstrated significant antioxidant activity, with a total phenolic content of 203.04 mg GAE/g DW and strong radical scavenging activity in DPPH (544.78 mg TE/g DW) and ABTS (312.15 mg TE/g DW) assays. In vitro assays showed marked cytotoxicity across multiple cancer cell lines, with IC50 values as low as 3.57 & micro;g/mL in A549 cells, indicating stronger activity compared to cisplatin. Flow cytometric analysis revealed substantial apoptosis induction (up to 53.67% in A549 cells) and G0/G1 cell cycle arrest (76.37% in A549). The extract also exhibited moderate antibacterial activity against E. coli and S. aureus, but no activity against P. aeruginosa or Candida species. These results position H. perforatum as a rich source of bioactive compounds with antioxidant, anticancer, and antimicrobial potential, and suggest further research into its therapeutic applications.
dc.description.sponsorshipInn niversitesi [4221]
dc.description.sponsorshipThis work was supported by the Scientific Research Projects Coordination Unit of Inonu University (ID: 4221).
dc.identifier.doi10.1038/s41598-026-36793-8
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.orcid0009-0004-3497-8397
dc.identifier.orcid0000-0002-9040-4249
dc.identifier.pmid41593177
dc.identifier.scopus2-s2.0-105030019397
dc.identifier.scopusqualityN/A
dc.identifier.urihttps://doi.org/10.1038/s41598-026-36793-8
dc.identifier.urihttps://hdl.handle.net/11616/109319
dc.identifier.volume16
dc.identifier.wosWOS:001691505300006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNature Portfolio
dc.relation.ispartofScientific Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectAntioxidant activity
dc.subjectApoptosis
dc.subjectCell cycle arrest
dc.subjectCytotoxicity
dc.subjectHypericum perforatum
dc.subjectLC-MS/MS
dc.subjectPhytochemical composition
dc.titleIntegrated chemical and biological characterization of Hypericum perforatum extract using LC-MS/MS and in vitro functional assays
dc.typeArticle

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