Does leflunomide attenuate the sepsis-induced acute lung injury?

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dc.authoridbegeç, zekine/0000-0002-9506-1871
dc.authoridErsoy, Mehmet/0000-0002-0724-2825
dc.authorwosidFadillioglu, Ersin/K-3817-2019
dc.authorwosidbegeç, zekine/ABI-5491-2020
dc.authorwosidErsoy, Mehmet/ACN-2779-2022
dc.contributor.authorOzturk, Erdogan
dc.contributor.authorDemirbilek, Semra
dc.contributor.authorBegec, Zekine
dc.contributor.authorSurucu, Murat
dc.contributor.authorFadillioglu, Ersin
dc.contributor.authorKirimhoglu, Hale
dc.contributor.authorErsoy, M. Ozcan
dc.date.accessioned2024-08-04T20:30:56Z
dc.date.available2024-08-04T20:30:56Z
dc.date.issued2008
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe organ that is affected first and most severely in intraabdominal sepsis is the lung. Oxygen radicals and active neutrophils in the lung are important sources for severe pulmonary inflammation leading to acute lung injury (ALI)/acute respiratory distress syndrome. The aim of this study was to investigate the effects of leflunomide, an immunomodulatory agent, on oxidant/antioxidant status with nitric oxide (NO) level and myeloperoxidase (MPO) activity in rats with sepsis-induced ALI. Fifty male Wistar albino rats were divided into five groups: control, sham, sepsis, leflunomide (10 mg/kg, intragastrically for two doses with an 8 h interval prior to the experiment) and sepsis + leflunomide. After the animals were anesthetized with ketamine and xylazine, the abdominal cavity was opened and ligated just below the ileocaecal valve with 3-0 silk. The antimesentric surface of the cecum was perforated and the cecum was gently compressed until fecal matter was extruded to induce sepsis. None of the rats received antibiotics during the experimental procedures. The experiment was ended 24 h after cecal ligation puncture (CLP) with the cervical dislocation under anesthesia. The lung tissues were removed for analysis of biochemical parameters and light microscopic investigation. The lung superoxide dismutase (SOD), catalase and glutathione peroxidase activities were decreased in the sepsis group as compared to the group control, sham, leflunomide and sepsis + leflunomide (P < 0.05), and SOD activity were significantly higher in group sepsis + leflunomide than sham, control, leflunomide and sepsis group (P < 0.05). The lung MPO, malondialdehyde (MDA), protein carbonyl and NO levels were higher in the sepsis group when compared to group control, sham, leflunomide and sepsis + leflunomide (P < 0.05), and MPO, MDA and NO levels were higher in the sepsis + leflunomide group than in the sham, control and leflunomide group (P < 0.05). The light microscopic evaluation showed that pulmonary architecture was preserved, and infiltration of neutrophil and edema decreased in sepsis + leflunomide group. The grade of alveolar damage was significantly decreased in sepsis + leflunomide group in comparison with sepsis group (P < 0.05). Our findings suggested that leflunomide attenuated the lung injury after CLP-induced sepsis by inhibition of neutrophils accumulation and increasing endogenous antioxidant capacity.en_US
dc.identifier.doi10.1007/s00383-008-2184-y
dc.identifier.endpage905en_US
dc.identifier.issn0179-0358
dc.identifier.issn1437-9813
dc.identifier.issue8en_US
dc.identifier.pmid18516612en_US
dc.identifier.scopus2-s2.0-48549101190en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage899en_US
dc.identifier.urihttps://doi.org/10.1007/s00383-008-2184-y
dc.identifier.urihttps://hdl.handle.net/11616/94612
dc.identifier.volume24en_US
dc.identifier.wosWOS:000258118800004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPediatric Surgery Internationalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectleflunomideen_US
dc.subjectacute lung injuryen_US
dc.subjectoxidative stressen_US
dc.subjectsepsisen_US
dc.titleDoes leflunomide attenuate the sepsis-induced acute lung injury?en_US
dc.typeArticleen_US

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