An effective VEGF-siRNA delivery via folic acid decorated and pegylated silica nanoparticles

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dc.authoridŞALVA, EMINE/0000-0002-1159-5850
dc.authoridTonbul, Hayrettin/0000-0001-5510-8973
dc.authoridUltav, Gozde/0000-0001-5582-3766
dc.authorwosidŞALVA, EMINE/CAH-3062-2022
dc.authorwosidTonbul, Hayrettin/AAR-6961-2020
dc.authorwosidUltav, Gözde/JPK-6247-2023
dc.contributor.authorUltav, Gozde
dc.contributor.authorTonbul, Hayrettin
dc.contributor.authorSalva, Emine
dc.date.accessioned2024-08-04T20:53:15Z
dc.date.available2024-08-04T20:53:15Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractLimitation of tumor vascularization can be helpful for cancer treatment. Silica nanoparticles can be produced in small diameters with good reproducibility, stability, and biocompatibility. Also, silica nanoparticles (SNPs) can be easily modified to carry negatively-charged oligonucleotides such as DNA or RNA and can be targeted to the tumor site by active targeting. In this study, we aimed to develop a gene delivery system exploiting the high amounts of folic acid receptors on breast cancer cells. Small-sized SNPs were synthesized and surface modifi-cations were performed by amination, PEGylation and folic acid conjugation (about 30 nm in diameter). Folic -acid conjugated SNPs (SNP-FA) complexed with VEGF-siRNA. Folic acid conjugation increased the cellular up-take by cancer cells (MDA-MB-231 and HeLa) according to the flow cytometer and fluorescence microscopy results. The VEGF gene silencing efficiency was determined by an ELISA and SNP-FA showed 73% and 50% gene silencing efficiency at HeLa and MDA-MB-231 cell lines, respectively. The results showed that SNPs with a suitable surface modification can be a good candidate for gene delivery.en_US
dc.description.sponsorshipTurkish Council of Science and Technology (TUBITAK); [118C470]en_US
dc.description.sponsorshipGraphical abstract was produced using Bio Render Software. TEM and SEM were performed in Middle East Technical University Central Laboratory. Special thanks to Prof. Koytepe and his team for FTIR equipment and Inonu University Hospital for Flow Cytometer equip- ment. And also special thanks to Ece Tavukcuoglu for helpful comments. A part of this study was supported by the Turkish Council of Science and Technology (TUBITAK) with the project code 118C470.en_US
dc.identifier.doi10.1016/j.jddst.2022.103828
dc.identifier.issn1773-2247
dc.identifier.issn2588-8943
dc.identifier.scopus2-s2.0-85144382399en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jddst.2022.103828
dc.identifier.urihttps://hdl.handle.net/11616/101070
dc.identifier.volume76en_US
dc.identifier.wosWOS:000869101100001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Drug Delivery Science and Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSilica nanoparticlesen_US
dc.subjectsiRNAen_US
dc.subjectGene deliveryen_US
dc.subjectFolic aciden_US
dc.subjectAnti-VEGFen_US
dc.titleAn effective VEGF-siRNA delivery via folic acid decorated and pegylated silica nanoparticlesen_US
dc.typeArticleen_US

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