A First Insight into Escherichia coli ST131 High-Risk Clone Among Extended-Spectrum Beta-Lactamase-Producing Urine Isolates in Istanbul with the Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry and Real-Time PCR

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dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridaktas, elif/0000-0003-3087-5425
dc.authorwosidaktas, elif/ACL-9792-2022
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.contributor.authorAktas, Elif
dc.contributor.authorOtlu, Baris
dc.contributor.authorErdemir, Duygu
dc.contributor.authorEkici, Hatice
dc.contributor.authorBulut, Emin
dc.date.accessioned2024-08-04T20:44:08Z
dc.date.available2024-08-04T20:44:08Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjectives: We aim to investigate, as a first insight, the presence and rates of high-risk Escherichia coli ST131 clone in Istanbul and evaluate antimicrobial resistance and CTX-M-15 production of ST131 and non-ST131 isolates. The use of MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry) to detect E. coli ST131 clone is also evaluated. Materials and Methods: A total of 203 extended-spectrum beta-lactamase (ESBL)-producing urinary isolates from a training hospital in Istanbul were investigated. Detection of E. coli ST131 was done by MALDI-TOF MS and real-time PCR melting curve analysis. The presence of CTX-M and CTX-M-15 beta-lactamases was investigated by PCR and sequence analysis. Results: Of the 203 isolates, 81 (39.9%) and 75 (36.9%) isolates were identified as ST131 clone by PCR and MALDI-TOF MS, respectively. Resistance to ciprofloxacin was significantly higher among ST131 isolates. A total of 169 (83.5%) isolates produced CTX-M beta-lactamase, of which 72 (43%) were CTX-M-15. The production of CTX-M and CTX-M-15 were significantly higher among ST131 isolates. Conclusions: We have demonstrated, for the first time, high rates of ST131 clone among ESBL-producing E. coli isolates in Istanbul, a region with high rates of resistance to third-generation cephalosporins and fluoroquinolones. Further investigation of this high-risk clone and its contribution to high antimicrobial resistance in Turkey is essential. MALDI-TOF MS is a useful tool for detection of high-risk clones and associated resistance patterns, simultaneous to bacterial identification.en_US
dc.identifier.doi10.1089/mdr.2017.0021
dc.identifier.endpage1036en_US
dc.identifier.issn1076-6294
dc.identifier.issn1931-8448
dc.identifier.issue8en_US
dc.identifier.pmid28467251en_US
dc.identifier.scopus2-s2.0-85037091418en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1032en_US
dc.identifier.urihttps://doi.org/10.1089/mdr.2017.0021
dc.identifier.urihttps://hdl.handle.net/11616/98057
dc.identifier.volume23en_US
dc.identifier.wosWOS:000416423300013en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofMicrobial Drug Resistanceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectST131en_US
dc.subjecthigh-risk cloneen_US
dc.subjectCTX-Men_US
dc.subjectCTX-M-15en_US
dc.subjectMALDI-TOF MSen_US
dc.subjectPCRen_US
dc.titleA First Insight into Escherichia coli ST131 High-Risk Clone Among Extended-Spectrum Beta-Lactamase-Producing Urine Isolates in Istanbul with the Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry and Real-Time PCRen_US
dc.typeArticleen_US

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