Effects of cholesterol and docosahexaenoic acid on cell viability and (Ca2+)i levels in acutely isolated mouse thymocytes

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dc.authorscopusid6602447584
dc.authorscopusid6504548872
dc.authorscopusid7006501958
dc.authorscopusid7201795413
dc.contributor.authorSandal S.
dc.contributor.authorTuneva J.
dc.contributor.authorYilmaz B.
dc.contributor.authorCarpenter D.O.
dc.date.accessioned2024-08-04T19:59:21Z
dc.date.available2024-08-04T19:59:21Z
dc.date.issued2009
dc.departmentİnönü Üniversitesien_US
dc.description.abstractWe investigated the effects of lipids on thymocyte function. The effects of application of cholesterol or docosahexaenoic acid (DHA), a C22, omega-3 (n-3) polyunsaturated fatty acid (PUFA), on viability and intracellular calcium concentrations of acutely isolated mouse thymocytes were investigated using flow cytometry. Cholesterol (100 ?M) caused significant cell death after 30-60 min whether or not calcium was present in the medium. Cell death was associated with an elevation of intracellular calcium whether or not calcium was present in the extracellular medium. However, the elevation of calcium concentration was not responsible for the cell death since calcium levels in the presence of ionomycin rose higher without significant cell death. DHA had similar actions but was more potent, causing significant cell death and elevation of calcium concentration within 5 min at 1 ?M. In the absence of extracellular calcium 1 ?M DHA caused 100% cell death within 15 min. Linolenic acid, a C18 omega-3 fatty acid also caused cytotoxicity at low concentrations whether or not albumin was present, but omega-6 or saturated C22 fatty acids were much less effective. These observations demonstrate that thymocyte viability is very sensitive to acute exposure to low concentrations of omega-3 fatty acids. Copyright © 2009 John Wiley & Sons, Ltd.en_US
dc.identifier.doi10.1002/cbf.1549
dc.identifier.endpage161en_US
dc.identifier.issn0263-6484
dc.identifier.issue3en_US
dc.identifier.pmid19274771en_US
dc.identifier.scopus2-s2.0-65549126900en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage155en_US
dc.identifier.urihttps://doi.org/10.1002/cbf.1549
dc.identifier.urihttps://hdl.handle.net/11616/90556
dc.identifier.volume27en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofCell Biochemistry and Functionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCell deathen_US
dc.subjectFlow cytometryen_US
dc.subjectFluo-3en_US
dc.subjectImmune functionen_US
dc.subjectPropidium iodideen_US
dc.titleEffects of cholesterol and docosahexaenoic acid on cell viability and (Ca2+)i levels in acutely isolated mouse thymocytesen_US
dc.typeArticleen_US

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